Possibility of a new biomarker in the diagnosis of primary aldosteronism

• Project/Area Number: 25460668
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hokkaido University
• Principal Investigator: JIN SHIGEKI 北海道大学, 保健科学研究院, 助教 (60531845)
• Co-Investigator(Renkei-kenkyūsha): CHIBA Hitoshi 北海道大学, 大学院保健科学研究院, 教授 (70197622) ; HUI Shu-Ping 北海道大学, 大学院保健科学研究院, 教授 (90337030)
• Research Collaborator: WADA Norio 市立札幌病院, 内分泌内科, 医師
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 臨床検査医学 / 原発性アルドステロン症 / バイオマーカー / 質量分析 / バイオマーカーの探索 / 化学合成 / 高速液体クロマトグラフィー / 固相抽出

Outline of Final Research Achievements

Primary aldosteronism is considered as approximately 10% of the causes of hypertension, so the diagnosis of its subtype is crucially important issue because cures are required on different kind of subtype. In this study, we found the substance which was detected more amount in the aldosterone-producing adenoma (APA) patient urine than that in the other subtypes of primary aldosteronism. We investigated the possibility of employing this substance as a new biomarker. The substance was detected as 1.5-2.0 fold amount in APA against essential hypertension and idiopathic hyperaldosteronism (IHA). However, its isolation and the structural analysis had not been executed until now.

Research Products

• [Journal Article] Quantification of urinary 18-hydroxycortisol using LC-MS/MS. (2013)
  • Author(s): Jin, S., Wada, N., Takahashi, Y., Hui, S-P., Sakurai, T., Fuda, H., Takeda, S., Fujikawa, M., Yanagisawa, K., Ikegawa, S., Kurosawa, T., Chiba, H.
  • Journal Title: Annals of Clinical Biochemistry Volume: 50 Issue: 5 Pages: 450-456
  • DOI: 10.1177/0004563213476272
  • Peer Reviewed
• [Presentation] Serum and urine 18-hydroxycortisol measured by LC-MS/MS in the diagnosis of primary aldosteronism and its subtypes. (2013)
  • Author(s): Norio WADA, Shigeki JIN, Shu-Ping HUI, Takao KUROSAWA, Hitoshi CHIBA
  • Organizer: International Symposium of Aldosterone and Related Substances in Hypertension 2013 (ISARSH2013)
  • Place of Presentation: Sendai International Center, Sendai, Japan