Comprehensive analyses and development of immunometric assay for plasmalogen

• Project/Area Number: 25460669
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hokkaido University
• Principal Investigator: Hui Shu-Ping 北海道大学, 保健科学研究院, 教授 (90337030)
• Co-Investigator(Kenkyū-buntansha): 千葉 仁志 北海道大学, その他の研究科, 教授 (70197622)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プラズマローゲン / plasmalogen / エタノールアミン型 / コリン型 / コリン型プラズマローゲン / エタノールアミン型プラズマローゲン / 化学合成 / 標準品

Outline of Final Research Achievements

Plasmalogens (Pls) are glycerophospholipids owning antioxidative effect, so it is conceivable that Pls may prevent arteriosclerosis and Alzheimer's disease. In our study, chemical synthesis method for choline Pls (PlsCho) has been improved; chemical synthesis method for ethanolamine Pls (PlsEtn) has been established.
Comprehensively qualitative and quantitative analyses of Pls in human serum have been carried out by LC-MS/MS using the synthetic Pls standards. The concentration ofPlsEtn18:0/20:4shows higher than that of PlsCho18:0/20:4 in human serum. Some mouses were immunized with BSA-plasmalogens obtained from chemical synthesis.

Research Products

• [Journal Article] Serum choline plasmalogens-those with oleic acid in sn-2-are biomarkers for coronary artery disease. (2014)
  • Author(s): Nishimukai, M., Maeba, R., Ikuta, A., Asakawa, N., Kamiya, K., Yamada, S., Yokota, T., Sakakibara, M., Tsutsui, H., Sakurai, T., Takahashi, Y., Hui, SP., Chiba, H., Okazaki, T., Hara, H.
  • Journal Title: Clinica Chimica Acta Volume: 437 Pages: 147-154
  • DOI: 10.1016/j.cca.2014.07.024
  • Peer Reviewed
• [Journal Article] Serum choline plasmalogens, particularly those with oleic acid in sn-2, are associated with proatherogenic state. (2014)
  • Author(s): Nishimukai M, Maeba R, Yamazaki Y, Nezu T, Sakurai T, Takahashi Y, Hui SP, Chiba H, Okazaki T, Hara H.
  • Journal Title: J Lipid Res Volume: 55 Issue: 5 Pages: 956-65
  • DOI: 10.1194/jlr.p045591
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Analysis of triacylglycerol hydroperoxides in human lipoproteins by Orbitrap mass spectrometer (2013)
  • Author(s): Hui SP et al.
  • Journal Title: Anal Bioanal Chem Volume: 405 Issue: 14 Pages: 4981-4987
  • DOI: 10.1007/s00216-013-6903-4
  • Peer Reviewed
• [Journal Article] Evaluation of various electrode materials for detection of oxidized low-density lipoproteins (2013)
  • Author(s): Takeda S, Hui SP, Fuda H, Jin S, Sakurai T, Ishii A, Mukasa K, Sueoka K, Chiba H.
  • Journal Title: J Biomed Nanotechnol Volume: 9 Pages: 303-6
  • Peer Reviewed
• [Journal Article] Quantification of urinary 18-hydroxycortisol using LC-MS/MS. (2013)
  • Author(s): Jin, S., Wada, N., Takahashi, Y., Hui, S-P., Sakurai, T., Fuda, H., Takeda, S., Fujikawa, M., Yanagisawa, K., Ikegawa, S., Kurosawa, T., Chiba, H.
  • Journal Title: Annals of Clinical Biochemistry Volume: 50 Issue: 5 Pages: 450-456
  • DOI: 10.1177/0004563213476272
  • Peer Reviewed
• [Journal Article] Immunological detection of large oxidized lipoproteins in hypertriglyceridemic serum (2013)
  • Author(s): Sakurai T, Wada N, Takahashi Y, Ichikawa A, Ikuta A, Furumaki H, Hui SP, Jin S, Takeda S, Fuda H, Fujikawa M, Shimizu C, Nagasaka H, Furukawa H, Kobayashi S, Chiba H.
  • Journal Title: Ann Clin Biochem Volume: 50 Issue: 5 Pages: 465-72
  • DOI: 10.1177/0004563212473446
  • Peer Reviewed
• [Journal Article] Measurement of single low-density lipoprotein particles by atomic force microscopy (2013)
  • Author(s): Sakurai T, Takeda S, Takahashi JY, Takahashi Y, Wada N, Trirongjitmoah S, Namita T, Jin S, Ikuta A, Furumaki H, Hui SP, Fuda H, Fujikawa M, Shimizu K, Chiba H.
  • Journal Title: Ann Clin Biochem Volume: 50 Issue: 6 Pages: 564-70
  • DOI: 10.1177/0004563213481586
  • Peer Reviewed
• [Presentation] 質量分析法による種々の脂質分子の定性及び定量分析 (2015)
  • Author(s): 惠 淑萍
  • Organizer: 第62回日本臨床検査医学会学術集会
  • Place of Presentation: 長良川国際会議場（岐阜県岐阜市）
  • Year and Date: 2015-11-20
• [Presentation] 脂肪滴の分析技術 (2015)
  • Author(s): 惠 淑萍
  • Organizer: 第22回日本未病システム学会学術総会
  • Place of Presentation: 北海道大学 学術交流会館(北海道札幌市）
  • Year and Date: 2015-10-11
• [Presentation] LC-MS/MSによるマガキ由来フェノール性抗酸化物質の定量 (2014)
  • Author(s): 岡部浩昭，惠 淑萍，池川繁男，比留間貴久，布田博敏，渡辺貢，千葉仁志
  • Organizer: 第54回日本臨床化学会年次学術集会
  • Place of Presentation: 東京 大学本郷キャンパス
  • Year and Date: 2014-09-05 – 2014-09-07
• [Presentation] 北海道大学高度脂質分析ラボの研究マネジメントと研究成果 (2014)
  • Author(s): 惠 淑萍
  • Organizer: 三重大学 2014年度 イノベーション創出セミナー
  • Place of Presentation: 三重大学
  • Year and Date: 2014-09-04
  • Invited
• [Presentation] Triglyceride-rich lipoproteins の過酸化脂質の分析 (2013)
  • Author(s): Rojeet Shrestha、惠 淑萍,八木亜希子、櫻井俊宏、高橋祐司、武田晴治、神 繁樹、千葉仁志
  • Organizer: 第38 回 日本医用マススペクトル学会年会
  • Place of Presentation: 神戸市産業振興センター （神戸市）
• [Presentation] 高度生殖医療に用いられるミネラルオイル中の過酸化脂質 (2013)
  • Author(s): 惠 淑萍 ・ 八木亜希子 ・ シュレスタロジート ・ 小林清一・ 千葉仁志 ・ 神谷博文
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: つくば国際会議場 エポカルつくば （つくば市）
• [Presentation] タンデム型質量分析計による臨床サンプルの定量分析 (2013)
  • Author(s): 惠 淑萍
  • Organizer: 第60回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸コンベンションセンター （神戸市）
• [Patent(Industrial Property Rights)] カプリン酸定量方法 (2013)
  • Inventor(s): 惠 淑萍、 平野賢一
  • Industrial Property Rights Holder: 惠 淑萍、 平野賢一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-224897
  • Filing Date: 2013-10-30