| Project/Area Number |
25460672
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Gunma University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Katsuyuki 群馬大学, 大学院医学系研究科, 非常勤講師 (10444051)
NAGAMINE Takeaki 群馬大学, 名誉教授 (90180520)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | メタロチオネイン-Ⅰ/Ⅱ / メタロチオネイン-Ⅲ / 前立腺がん / ウィルソン病 / メンケス病 / ELISA / メタロチオネイン-I/II / メタロチオネイン-III / ＥＬＩＳＡ |
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| Outline of Final Research Achievements |
We developed a new competitive ELISA for determination of metallothionein (MT)-Ⅲ. However, it was more difficult to measure MT-Ⅲ in human serum, and further efforts to improve the assay system for MT-Ⅲ determination in serum is now undergoing in our laboratory.
In the study using MT-Ⅰ/Ⅱ antibody and ELISA, the MT-Ⅰ/Ⅱ level was significantly reduced in the liver with NASH compared with NAFLD and control. The serum and urine MT-Ⅰ/Ⅱ levels in prostate cancer patients were significantly higher than healthy controls. The serum MT-Ⅰ/Ⅱ levels in Wilson's disease and Menkes disease patients were significantly higher than healthy controls, and similar result was found in LEC rat (model animal of Wilson's disease) and Macular mouse (model animal of Menken disease). In conclusion, the measurement of serum MT-Ⅰ/Ⅱ is suggested to be a potentially useful diagnostic tool in patients with some diseases, particularly Wilson's disease or Menkes disease.
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