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Clathrin Heavy chain is a potential biomarker for histological diagnosis of various cancer

Research Project

Project/Area Number 25460675
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionChiba University

Principal Investigator

SEIMIYA Masanori  千葉大学, 医学部附属病院, 副臨床検査技師長 (20554265)

Co-Investigator(Kenkyū-buntansha) NOMURA Fumio  千葉大学, 医学部附属病院, 特任教授 (80164739)
MATSUSHITA Kazuyuki  千葉大学, 大学院医学研究院, 准教授 (90344994)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords臨床検査 / 病理診断 / クラスリン重鎖 / 免疫組織化学 / clathrin heavy chain / p53 / ki67 / Clathrin heavy chain / 原発性肝細胞癌 / 食道癌 / 免疫組織化学染色 / 病理組織診断 / 腫瘍マーカー
Outline of Final Research Achievements

Clathrin Heavy Chain (CHC) is an endocytosis related protein. We previously reported the immunohistochemical staining of CHC as a histological diagnostic marker of liver cancer or esophageal cancer. In this study, the usefulness for the histopathological diagnosis of pharynx carcinoma, larynx carcinoma, tongue carcinoma, and gingiva carcinoma were investigated. CHC was significantly more strongly expressed in the cytoplasm of malignant legions, than in that of normal tissue or benign lesions. Therefore, the immunohistochemical staining of CHC is a potential biomarker for histological diagnosis of these cancer. Moreover, we synthesized the 12 peptide for serum anti-CHC antibody level. It will be necessary to develop the ELISA method and to evaluate performance for diagnosis of various cancer.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Paradoxical effects of thyroid function on glomerular filtration rate estimated from serum creatinine or standardized cystatin C in patients with Japanese Graves' disease2015

    • Author(s)
      Suzuki Y, Matsushita K, Seimiya M, Yoshida T, Sawabe Y, Ogawa M, Nomura F
    • Journal Title

      Clin Chim Acta

      Volume: 7 Pages: 316-322

    • DOI

      10.1016/j.cca.2015.10.018

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimated glomerular filtration rate by serum creatinine or standardized cystatin C in Japanese patients with Graves disease2015

    • Author(s)
      Suzuki Y, Matsushita K, Seimiya M, Yoshida T, Sawabe Y, Ogawa M, Nomura F
    • Journal Title

      Data Brief

      Volume: 24 Pages: 1092-1095

    • DOI

      10.1016/j.dib.2015.11.023

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The abnormal reaction data-detecting function of the automated biochemical analyzer was useful to prevent erroneous total-bilirubin measurement and to identify monoclonal proteins2015

    • Author(s)
      Seimiya M, Suzuki Y, Yoshida T, Sawabe Y, Matsushita K, Nomura F
    • Journal Title

      Clinica Chimica Acta

      Volume: 441 Pages: 44-46

    • DOI

      10.1016/j.cca.2014.12.013

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] サイトケラチン19フラグメント (CYFRA) の物理的刺激による負の影響についての検討2015

    • Author(s)
      万場真乃介, 清宮正徳, 吉田俊彦, 澤部祐司, 野村文夫
    • Journal Title

      千臨技会誌 (123): 23-26, 2015.

      Volume: 123 Pages: 23-26

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Change in albumin measurement method affects diagnosis of nephrotic syndrome2014

    • Author(s)
      Seimiya M, Ohno S, Asano H, Fujiwara K, Yoshida T, Sawabe Y, Sogawa K, Ogawa M, Matsushita K, Yokosuka O, Nomura F
    • Journal Title

      Clinical Laboratory

      Volume: 60 Pages: 1663-1667

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway2014

    • Author(s)
      Rahmutulla B, Matsushita K, Satoh M, Seimiya M, Tsuchida S, Kubo S, Shimada H, Ohtsuka M, Miyazaki M, Nomura F
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 2404-2417

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway2014

    • Author(s)
      Bahityar Rahmutulla, Kazuyuki Matsushita, Mamoru Satoha, Masanori Seimiya, Sachio Tsuchida, Shuji Kubo, Hideaki Shimada, Masayuki Otsuka, Masaru Miyazaki, Fumio Nomura
    • Journal Title

      Oncotarget

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Determination of Serum Carbohydrate-Deficient Transferrin (CDT) by the Nephelometric N Latex CDT Assay in Japanese Habitual Drinkers and Patients with Non-Alcoholic Liver Diseases2014

    • Author(s)
      Mako Yamada, Masanori Seimiya, Mamoru Satoh, Sakae Itoga, Kazuyuki Sogawa, Hirotaka Takizawa, Osamu Yokosuka, Fumio Nomura
    • Journal Title

      Alcoholism & Drug Dependence

      Volume: 1 Pages: 1000138-1000138

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Change in albumin measurement method affects diagnosis of nephrotic syndrome2014

    • Author(s)
      Masanori SEIMIYA, Sayaka OHNO, Haruna ASANO, Keiko FUJIWARA, Toshihiko YOSHIDA, Yuji SAWABE, Kazuyuki SOGAWA, Makoto OGAWA, Kazuyuki MATSUSHITA, Osamu YOKOSUKA, Fumio NOMURA
    • Journal Title

      Clinical Laboratory

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clathrin heavy chain is a useful immunohistochemical marker for esophageal squamous intraepithelial neoplasia.2013

    • Author(s)
      Tokita K, Seimiya M, Matsushita K, Tomonaga T, Onodera K, Ohki S, Tohru T, Uesato M, Shimada H, Matsubara H, Nakatani Y, Nomura F.
    • Journal Title

      Esophagus

      Volume: - Issue: 4 Pages: 1-6

    • DOI

      10.1007/s10388-013-0381-9

    • NAID

      40019956893

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Preanalytical evaluation of serum 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 measurements using LC-MS/MS.2013

    • Author(s)
      Mochizuki A, Kodera Y, Saito T, Satoh M, Sogawa K, Nishimura M, Seimiya M,Kubota M, Nomura F.
    • Journal Title

      Clin Chim Acta.

      Volume: 420 Pages: 114-120

    • DOI

      10.1016/j.cca.2012.10.041

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Combined proteomic analysis of liver tissue and serum in chronically alcohol-fed rats2013

    • Author(s)
      Yamada M, Satoh M, Seimiya M, Sogawa K, Itoga S, Tomonaga T, Nomura F
    • Journal Title

      Alcohol Clin Exp Res

      Volume: 37 Issue: s1

    • DOI

      10.1111/j.1530-0277.2012.01883.x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Child-pugh score is altered by the albumin measurement method2013

    • Author(s)
      Seimiya M, Ohno S, Yamamoto H, Fujiwara K, Yoshida T, Sawabe Y, Sogawa K, Matsushita K, Yokosuka O, Nomura F
    • Journal Title

      Hepatology

      Volume: 57(5) Issue: 5 Pages: 2093-4

    • DOI

      10.1002/hep.25972

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Usefulness of abnormal reaction detection system of automated biochemical analyzer2015

    • Author(s)
      Seimiya M, Suzuki Y, Yoshida T, Sawabe Y, Matsushita K, Nomura F
    • Organizer
      EUROMEDLAB PARIS
    • Place of Presentation
      Palais des Congres, パリ(フランス)
    • Year and Date
      2015-06-21 – 2015-06-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Usefulness of abnormal reaction detection system of automated biochemical analyzer2015

    • Author(s)
      Masanori Seimiya, Fumio Nomura, et al
    • Organizer
      21st IFCC - EFLM European Congress of Clinical Chemistry and Laboratory Medicine
    • Place of Presentation
      パリ(フランス)
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] これからの臨床化学検査に求められること2014

    • Author(s)
      清宮正徳
    • Organizer
      東京・千葉・埼玉合同臨床化学検査研究班研修会
    • Place of Presentation
      東京医科歯科大学, 東京都文京区
    • Year and Date
      2014-07-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] The abnormal reaction data-detecting function of the automated biochemical analyzer is useful to prevent the reporting of erroneous test values and discovery of abnormal proteins in patients.2014

    • Author(s)
      Seimiya M, Suzuki Y, Yoshida T, Sawabe Y, Matsushita K, Nomura F
    • Organizer
      International Congress of Clinical Chemistry and Laboratory Medicine
    • Place of Presentation
      Istanbul Congress Center, イスタンブール(トルコ)
    • Year and Date
      2014-06-22 – 2014-06-26
    • Related Report
      2014 Research-status Report
  • [Presentation] The abnormal reaction data-detecting function of the automated biochemical analyzer was useful to prevent erroneous total-bilirubin measurement and to identify monocloncal proteins2014

    • Author(s)
      Seimiya Masanori, Yoshida toshihiko, Sawabe yuji, Nomura Fumio
    • Organizer
      IFCC
    • Place of Presentation
      Turkey Istanbul
    • Related Report
      2013 Research-status Report
  • [Presentation] Change in albumin measurement method affects diagnosis of nephrotic syndrome2013

    • Author(s)
      Seimiya Masanori, Yoshida toshihiko, Sawabe yuji, Nomura Fumio
    • Organizer
      13th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine
    • Place of Presentation
      Indonesia Bali
    • Related Report
      2013 Research-status Report
  • [Presentation] クラスリン重鎖の免疫組織化学染色は食道粘膜内腫瘍の病理組織診断に有用である2013

    • Author(s)
      清宮正徳, 時田和也, 松下一之, 朝長毅, 谷澤徹, 上里昌也, 島田英昭, 松原久裕, 中谷行雄, 野村文夫
    • Organizer
      日本分子腫瘍マーカー研究会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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