Clathrin Heavy chain is a potential biomarker for histological diagnosis of various cancer

• Project/Area Number: 25460675
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: SEIMIYA Masanori 千葉大学, 医学部附属病院, 副臨床検査技師長 (20554265)
• Co-Investigator(Kenkyū-buntansha): NOMURA Fumio 千葉大学, 医学部附属病院, 特任教授 (80164739) ; MATSUSHITA Kazuyuki 千葉大学, 大学院医学研究院, 准教授 (90344994)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 臨床検査 / 病理診断 / クラスリン重鎖 / 免疫組織化学 / clathrin heavy chain / p53 / ki67 / Clathrin heavy chain / 原発性肝細胞癌 / 食道癌 / 免疫組織化学染色 / 病理組織診断 / 腫瘍マーカー

Outline of Final Research Achievements

Clathrin Heavy Chain (CHC) is an endocytosis related protein. We previously reported the immunohistochemical staining of CHC as a histological diagnostic marker of liver cancer or esophageal cancer. In this study, the usefulness for the histopathological diagnosis of pharynx carcinoma, larynx carcinoma, tongue carcinoma, and gingiva carcinoma were investigated. CHC was significantly more strongly expressed in the cytoplasm of malignant legions, than in that of normal tissue or benign lesions. Therefore, the immunohistochemical staining of CHC is a potential biomarker for histological diagnosis of these cancer. Moreover, we synthesized the 12 peptide for serum anti-CHC antibody level. It will be necessary to develop the ELISA method and to evaluate performance for diagnosis of various cancer.

Research Products

• [Journal Article] Paradoxical effects of thyroid function on glomerular filtration rate estimated from serum creatinine or standardized cystatin C in patients with Japanese Graves' disease (2015)
  • Author(s): Suzuki Y, Matsushita K, Seimiya M, Yoshida T, Sawabe Y, Ogawa M, Nomura F
  • Journal Title: Clin Chim Acta Volume: 7 Pages: 316-322
  • DOI: 10.1016/j.cca.2015.10.018
  • Peer Reviewed
• [Journal Article] Estimated glomerular filtration rate by serum creatinine or standardized cystatin C in Japanese patients with Graves disease (2015)
  • Author(s): Suzuki Y, Matsushita K, Seimiya M, Yoshida T, Sawabe Y, Ogawa M, Nomura F
  • Journal Title: Data Brief Volume: 24 Pages: 1092-1095
  • DOI: 10.1016/j.dib.2015.11.023
  • Peer Reviewed
• [Journal Article] The abnormal reaction data-detecting function of the automated biochemical analyzer was useful to prevent erroneous total-bilirubin measurement and to identify monoclonal proteins (2015)
  • Author(s): Seimiya M, Suzuki Y, Yoshida T, Sawabe Y, Matsushita K, Nomura F
  • Journal Title: Clinica Chimica Acta Volume: 441 Pages: 44-46
  • DOI: 10.1016/j.cca.2014.12.013
  • Peer Reviewed
• [Journal Article] サイトケラチン19フラグメント (CYFRA) の物理的刺激による負の影響についての検討 (2015)
  • Author(s): 万場真乃介, 清宮正徳, 吉田俊彦, 澤部祐司, 野村文夫
  • Journal Title: 千臨技会誌 (123): 23-26, 2015. Volume: 123 Pages: 23-26
  • Peer Reviewed
• [Journal Article] Change in albumin measurement method affects diagnosis of nephrotic syndrome (2014)
  • Author(s): Seimiya M, Ohno S, Asano H, Fujiwara K, Yoshida T, Sawabe Y, Sogawa K, Ogawa M, Matsushita K, Yokosuka O, Nomura F
  • Journal Title: Clinical Laboratory Volume: 60 Pages: 1663-1667
  • Peer Reviewed
• [Journal Article] Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway (2014)
  • Author(s): Rahmutulla B, Matsushita K, Satoh M, Seimiya M, Tsuchida S, Kubo S, Shimada H, Ohtsuka M, Miyazaki M, Nomura F
  • Journal Title: Oncotarget Volume: 5 Pages: 2404-2417
  • Peer Reviewed / Open Access
• [Journal Article] Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway (2014)
  • Author(s): Bahityar Rahmutulla, Kazuyuki Matsushita, Mamoru Satoha, Masanori Seimiya, Sachio Tsuchida, Shuji Kubo, Hideaki Shimada, Masayuki Otsuka, Masaru Miyazaki, Fumio Nomura
  • Journal Title: Oncotarget Volume: in press
  • Peer Reviewed
• [Journal Article] Determination of Serum Carbohydrate-Deficient Transferrin (CDT) by the Nephelometric N Latex CDT Assay in Japanese Habitual Drinkers and Patients with Non-Alcoholic Liver Diseases (2014)
  • Author(s): Mako Yamada, Masanori Seimiya, Mamoru Satoh, Sakae Itoga, Kazuyuki Sogawa, Hirotaka Takizawa, Osamu Yokosuka, Fumio Nomura
  • Journal Title: Alcoholism & Drug Dependence Volume: 1 Pages: 1000138-1000138
  • Peer Reviewed
• [Journal Article] Change in albumin measurement method affects diagnosis of nephrotic syndrome (2014)
  • Author(s): Masanori SEIMIYA, Sayaka OHNO, Haruna ASANO, Keiko FUJIWARA, Toshihiko YOSHIDA, Yuji SAWABE, Kazuyuki SOGAWA, Makoto OGAWA, Kazuyuki MATSUSHITA, Osamu YOKOSUKA, Fumio NOMURA
  • Journal Title: Clinical Laboratory Volume: in press
  • Peer Reviewed
• [Journal Article] Clathrin heavy chain is a useful immunohistochemical marker for esophageal squamous intraepithelial neoplasia. (2013)
  • Author(s): Tokita K, Seimiya M, Matsushita K, Tomonaga T, Onodera K, Ohki S, Tohru T, Uesato M, Shimada H, Matsubara H, Nakatani Y, Nomura F.
  • Journal Title: Esophagus Volume: - Issue: 4 Pages: 1-6
  • DOI: 10.1007/s10388-013-0381-9
  • NAID: 40019956893
  • Peer Reviewed
• [Journal Article] Preanalytical evaluation of serum 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 measurements using LC-MS/MS. (2013)
  • Author(s): Mochizuki A, Kodera Y, Saito T, Satoh M, Sogawa K, Nishimura M, Seimiya M,Kubota M, Nomura F.
  • Journal Title: Clin Chim Acta. Volume: 420 Pages: 114-120
  • DOI: 10.1016/j.cca.2012.10.041
  • Peer Reviewed
• [Journal Article] Combined proteomic analysis of liver tissue and serum in chronically alcohol-fed rats (2013)
  • Author(s): Yamada M, Satoh M, Seimiya M, Sogawa K, Itoga S, Tomonaga T, Nomura F
  • Journal Title: Alcohol Clin Exp Res Volume: 37 Issue: s1
  • DOI: 10.1111/j.1530-0277.2012.01883.x
  • Peer Reviewed
• [Journal Article] Child-pugh score is altered by the albumin measurement method (2013)
  • Author(s): Seimiya M, Ohno S, Yamamoto H, Fujiwara K, Yoshida T, Sawabe Y, Sogawa K, Matsushita K, Yokosuka O, Nomura F
  • Journal Title: Hepatology Volume: 57(5) Issue: 5 Pages: 2093-4
  • DOI: 10.1002/hep.25972
  • Peer Reviewed
• [Presentation] Usefulness of abnormal reaction detection system of automated biochemical analyzer (2015)
  • Author(s): Seimiya M, Suzuki Y, Yoshida T, Sawabe Y, Matsushita K, Nomura F
  • Organizer: EUROMEDLAB PARIS
  • Place of Presentation: Palais des Congres, パリ（フランス）
  • Year and Date: 2015-06-21 – 2015-06-25
• [Presentation] Usefulness of abnormal reaction detection system of automated biochemical analyzer (2015)
  • Author(s): Masanori Seimiya, Fumio Nomura, et al
  • Organizer: 21st IFCC - EFLM European Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: パリ（フランス）
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] これからの臨床化学検査に求められること (2014)
  • Author(s): 清宮正徳
  • Organizer: 東京・千葉・埼玉合同臨床化学検査研究班研修会
  • Place of Presentation: 東京医科歯科大学, 東京都文京区
  • Year and Date: 2014-07-05
  • Invited
• [Presentation] The abnormal reaction data-detecting function of the automated biochemical analyzer is useful to prevent the reporting of erroneous test values and discovery of abnormal proteins in patients. (2014)
  • Author(s): Seimiya M, Suzuki Y, Yoshida T, Sawabe Y, Matsushita K, Nomura F
  • Organizer: International Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: Istanbul Congress Center, イスタンブール（トルコ）
  • Year and Date: 2014-06-22 – 2014-06-26
• [Presentation] The abnormal reaction data-detecting function of the automated biochemical analyzer was useful to prevent erroneous total-bilirubin measurement and to identify monocloncal proteins (2014)
  • Author(s): Seimiya Masanori, Yoshida toshihiko, Sawabe yuji, Nomura Fumio
  • Organizer: IFCC
  • Place of Presentation: Turkey Istanbul
• [Presentation] Change in albumin measurement method affects diagnosis of nephrotic syndrome (2013)
  • Author(s): Seimiya Masanori, Yoshida toshihiko, Sawabe yuji, Nomura Fumio
  • Organizer: 13th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine
  • Place of Presentation: Indonesia Bali
• [Presentation] クラスリン重鎖の免疫組織化学染色は食道粘膜内腫瘍の病理組織診断に有用である (2013)
  • Author(s): 清宮正徳, 時田和也, 松下一之, 朝長毅, 谷澤徹, 上里昌也, 島田英昭, 松原久裕, 中谷行雄, 野村文夫
  • Organizer: 日本分子腫瘍マーカー研究会
  • Place of Presentation: 横浜