| Project/Area Number |
25460678
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hokuriku University (2015)
Shinshu University (2013-2014) |
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Principal Investigator |
Terasawa Fumiko 北陸大学, 公私立大学の部局等, 教授 (40109210)
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| Co-Investigator(Kenkyū-buntansha) |
奥村 伸生 信州大学, 医学部, 教授 (60252110)
石井 亘 信州大学, 医学部, 講師 (80452142)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | シトルリン化フィブリノゲン / ELISA / 関節リウマチ / 血中抗原濃度 / 血中抗体濃度 / 至適条件 / ビオチン化抗ヒトIgG / ストレプトアビジン-POD |
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| Outline of Final Research Achievements |
We measured citrullinated fibrinogen and autoantibody levels in the serum using ELISA based on recombinant citrullinated fibrinogen and a mouse monoclonal antibody against it.
The citrullinated fibrinogen levels of the rheumatoid arthritis (RA) patients (n=13), systemic lupus erythematosus (SLE) patients (n=2), and normal controls (n=21) were 204-2881 ng/mL, 92ng/mL, and 47-291 ng/ml (134±60.1 ng/mL), respectively. The citrullinated fibrinogen antibody levels of the RA patients, SLE patients, and normal controls were 25-1,780 U/mL, 20U/mL, and 4.0-10.9 U/mL (5.4±1.54 U/mL), respectively. The serum measurements obtained in the RA patients indicated that the assay exhibited 84.6% sensitivity and 95.7% specificity for the citrullinated fibrinogen antigen, and 100% sensitivity and 87.0% specificity for the citrullinated fibrinogen antibody.
In this study, we established an ELISA-based method for measuring the serum levels of citrullinated fibrinogen and antibodies against it.
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