Development of a novel chromogenic method, Penta-well test, for rapid prediction of beta-lactamase classes produced in clinical Enterobacteriaceae isolates
| Project/Area Number |
25460681
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
Kawamura Kumiko 名古屋大学, 医学(系)研究科(研究院), 准教授 (30335054)
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| Co-Investigator(Kenkyū-buntansha) |
YAGI TETSUYA 名古屋大学, 大学院医学系研究科, 教授 (70333573)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAKAWA YOSHICHIKA 名古屋大学, 大学院医学系研究科, 教授 (10212622)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | グラム陰性桿菌 / β-ラクタマーゼ / 複数酵素同時産生菌 / 新規検出法 / β-ラクタマーゼ産生性の確認 / β-ラクタマーゼのクラス分類 |
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| Outline of Final Research Achievements |
We developed a novel chromogenic method, Penta-well test, which enables the rapid detection and classification of β-lactamases in clinical Enterobacteriaceae isolates. This test is based on a combination of nitrocefin and three β-lactamase inhibitors specific to class A, B, and/or C, with nitrocefin hydrolysis by β-lactamases. When the cut-off value for each β-lactamase class was determined (0.25, 0.4, and 0.55 for class A, class B, and class C β-lactamase producers, respectively), the sensitivity and specificity of classification were 96.3% and 97.1% for class A, 97.4% and 100% for class B, and 85.7% and 100% for class C, respectively. Moreover, this method allowed accurate β-lactamase classification in 21 of 23 (91.3%) isolates producing plural class of β-lactamases. Thus, the Penta-well test can provide information that would be useful in the accurate detection and classification of β-lactamases produced by causative bacteria.
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Report
(4 results)
Research Products
(11 results)
