Stool DNA testing for detection of colorectal tumors

• Project/Area Number: 25460687
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Yamaguchi University
• Principal Investigator: SUEHIRO Yutaka 山口大学, 医学(系)研究科(研究院), 准教授 (40290978)
• Co-Investigator(Kenkyū-buntansha): OKAYAMA Naoko 山口大学, 医学部附属病院・副臨床, 衛生検査技師長 (40420541)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 大腸癌 / 便DNA検査 / メチル化

Outline of Final Research Achievements

As TWIST1 methylation is specific to colorectal tumors, detection of TWIST1 methylation from stool samples might be useful for colorectal tumor screening. However, because the content of human DNA in stool is very small, it is very difficult to detect TWIST1 methylation by conventional bisulfite-based methylation assays. Therefore, we developed a new methylation assay without bisulfite treatment.

Research Products

• [Journal Article] Highly sensitive stool DNA testing of Fusobacterium nucleatum as a marker for detection of colorectal tumours in a Japanese population. (2016)
  • Author(s): Suehiro Y, Sakai K, Nishioka M, Hashimoto S, Takami T, Higaki S, Shindo Y, Hazama S, Oka M, Nagano H, Sakaida I, Yamasaki T.
  • Journal Title: Ann Clin Biochem Volume: April 28 Issue: 1 Pages: 86-91
  • DOI: 10.1177/0004563216643970
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Long-fragment DNA as a potential marker for stool-based detection of colorectal cancer (2015)
  • Author(s): YIBO ZHANG, YUTAKA SUEHIRO, YOSHITARO SHINDO, KOUHEI SAKAI, SHOICHI HAZAMA, SHINGO HIGAKI, ISAO SAKAIDA, MASAAKI OKA, TAKAHIRO YAMASAKI
  • Journal Title: Oncology Letters Volume: 9 Issue: 1 Pages: 454-458
  • DOI: 10.3892/ol.2014.2632
  • Peer Reviewed / Open Access
• [Patent(Industrial Property Rights)] 大腸腫瘍の有無の予測を補助する方法 (2016)
  • Inventor(s): 末廣 寛、山﨑 隆弘
  • Industrial Property Rights Holder: 山口大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-024772
  • Filing Date: 2016-02-12
• [Patent(Industrial Property Rights)] 大腸腫瘍の有無を検査する方法 (2015)
  • Inventor(s): 末廣 寛
  • Industrial Property Rights Holder: 山口大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-177040
  • Filing Date: 2015-09-08