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Development of high sensitivity antibody protein chip using tagged antibody produced by genetic recombination technology.

Research Project

Project/Area Number 25460689
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionYamaguchi University

Principal Investigator

FURUMOTO Hiroko  山口大学, 医学(系)研究科(研究院), 講師 (70311818)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords蛋白チップ / 抗体開発 / タンパクチップ
Outline of Final Research Achievements

An antibody chip is a tool used to exhaustively analyze protein expression in full detail. The purpose of this study is to create a new technology for antibody arrays by developing a highly sensitive next generation antibody chip, using the technology where a tagged protein is immobilized on a diamond-like surface treated substrate. Thus, we created a monoclonal antibody where the tag-sequence was added to immunoglobulin G (IgG) using genetic recombination technology. The tag introduced IgG and a control IgG were produced in a cell culture system. Then, they were fixed to the substrate to evaluate the state of the antibody and antigen-detection efficiency on the substrate with or without the tag. As a result, it was confirmed that a tagged-antibodies are high sensitivity, but hundreds of different kind of tagged-antibodies are required for the creation of the antibody array. Therefore, a high-throughput production system of the tagged-antibody, such as genome editing is under trial.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2013

All Presentation (1 results)

  • [Presentation] Protein-protein interaction analysis using oligo-cysteine tagged protein chip. Detection of tubulin from mouse brain extract by oligo-cysteine tagged stathmin chip.2013

    • Author(s)
      古元 礼子、清水真央子、田中寿幸、北川孝雄、赤田純子、中村和行
    • Organizer
      HUPO2013
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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