Development of high sensitivity antibody protein chip using tagged antibody produced by genetic recombination technology.

Research Project

Project/Area Number	25460689
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Laboratory medicine
Research Institution	Yamaguchi University
Principal Investigator	FURUMOTO Hiroko 山口大学, 医学(系)研究科(研究院), 講師 (70311818)
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	蛋白チップ / 抗体開発 / タンパクチップ
Outline of Final Research Achievements	An antibody chip is a tool used to exhaustively analyze protein expression in full detail. The purpose of this study is to create a new technology for antibody arrays by developing a highly sensitive next generation antibody chip, using the technology where a tagged protein is immobilized on a diamond-like surface treated substrate. Thus, we created a monoclonal antibody where the tag-sequence was added to immunoglobulin G (IgG) using genetic recombination technology. The tag introduced IgG and a control IgG were produced in a cell culture system. Then, they were fixed to the substrate to evaluate the state of the antibody and antigen-detection efficiency on the substrate with or without the tag. As a result, it was confirmed that a tagged-antibodies are high sensitivity, but hundreds of different kind of tagged-antibodies are required for the creation of the antibody array. Therefore, a high-throughput production system of the tagged-antibody, such as genome editing is under trial.