| Project/Area Number |
25460692
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Takajo Ichiro 宮崎大学, 医学部, 講師 (20418841)
Hayashi Tetsuya 九州大学, 医学研究院, 教授 (10173014)
Koshimoto Chihiro 宮崎大学, フロンティア実験総合センター, 教授 (70295210)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 感染症 / 新規診断法 / レーザーマイクロダイセクション / レーザーマイクロダイセクション法 |
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| Outline of Final Research Achievements |
To identify the causative agents for bacterial infection, we tried to apply the micro laser dissection (LMD) to obtain the bacterial DNA from the gram stained slides in combination to determine its DNA sequences from PCR product. When Escherichia coli were used as its subject, even 100 bacteria can be successfully identified using primers for 16S ribosomal RNA genome. However, the contaminated bacterial genome from circumstance was also detected when the sensitivity of this method was increased. Therefore, we also tried to develop PCR pathogen-specific primers and obtained good sensitivity and specificity. We are planning to perform animal experiment and apply this method for the clinical samples.
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