| Project/Area Number |
25460698
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyorin University |
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Principal Investigator |
AISO TOSHIKO 杏林大学, 保健学部, 教授 (40195144)
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| Co-Investigator(Kenkyū-buntansha) |
大西 宏明 杏林大学, 医学部, 教授 (80291326)
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| Co-Investigator(Renkei-kenkyūsha) |
GAMOU SHINOBU 杏林大学, CCRC研究所, 教授 (90122308)
HIGASHI KATSUMI 杏林大学, 保健学部, 特任教授 (50159109)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | マイクロRNA / バイオマーカー / 肺がん / リアルタイムPCR / microRNA / 非小細胞肺がん / 血中マイクロRNA / circulating microRNA / 血清 |
|---|
| Outline of Final Research Achievements |
We analyzed circulating microRNAs miR-20a, miR-21, miR-145 and miR-223 as candidate markers of lung cancer: (1) miR-145 was degraded rapidly in serum samples at 4°C; the level dropped to 29.3% over 24 h. MiR-145 in extracellular vesicles exhibited similar instability; the level was 56.5%; (2) The level of microRNA miR-21 in serum decreased 44% in healthy passive smokers. (3) On two of these four microRNAs, the levels decreased significantly in serum of non-small cell lung cancer patients. Moreover, these levels in serum increased after the excision of the cancer.
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