The research for the susceptibility genes of multifactorial genetic disease applied bottom-up proteomics

Research Project

Project/Area Number	25460703
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Laboratory medicine
Research Institution	Nihon University
Principal Investigator	NAKAYAMA Tomohiro 日本大学, 医学部, 教授 (00339334)
Co-Investigator(Kenkyū-buntansha)	KUBOTA Takeo 山梨大学, 総合研究部医学, 教授 (70293511) NOMURA Fumio 千葉大学, 医学(系)研究科(研究院), 教授 (80164739)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	遺伝性疾患 / プロテオミクス / 感受性遺伝子 / 疾患マーカー / 遺伝子 / 塩基配列決定法
Outline of Final Research Achievements	We have collected the seventy-eight samples with essential hypertension, cerebral infarction, myocardial infarction, diabetes. After getting a written consent, we extracted genomic DNA from white blood cells. We inspected participants whether they have family history of the disorders by hearing exact information about family careers. The clinical data such as sex, age, family history, height, weight, body mass index, total cholesterol, triglyceride, HDL cholesterol, uric acid, blood sugar, urine acids, electrocardiogram, a smoking history, drinking career. The DNA samples were stocked in deep freezers until analysis, and the isolated plasma were analyzed for proteomics analysis by the MALDI-TOF MS. A next-generation sequencer was used for the genomics analysis especially for exome analysis. In addition, Sanger method was applied for the individual genetic analysis. SNPAlyze software was used for haplotype-based case-control study.