| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We developed remarkably simple enzymatic assays based on a signal accumulation type of ion-sensitive field-effect transistor (SA-ISFET) technology. Decreases in proton concentration resulting from enzyme reactions can be directly and effectively detected in real time by SA-ISFET as a change in potential. The assay methods exhibited high sensitivity, linearity, and reproducibility when potential signals are accumulated 10-fold. We also developed a simple and compact-sized analyzer using the biosensor equipped with SA-ISFET. The proton quantities increased or decreased by enzyme reactions with small amount of samples were directly detected by the SA-ISFET sensor as altered potentials, whereas conventional enzymatic assays required labeling process or coloring process. The biosensor was further improved with built-in reference electrodes and capacities optimized reaction chambers. In future, this analyzer can be applied to various measurement systems based on enzyme reactions.
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