Generation of "antibody-like" proteins based on evolutionary molecular engineering for novel clinical diagnostics
Project/Area Number |
25460709
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Kobe Pharmaceutical University |
Principal Investigator |
MORITA IZUMI 神戸薬科大学, 薬学部, 助手 (20299085)
|
Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Norihiro 神戸薬科大学, 薬学部, 教授 (90205477)
OYAMA Hiroyuki 神戸薬科大学, 薬学部, 助教 (80572966)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 進化分子工学 |
Outline of Final Research Achievements |
Antibodies are widely used in various clinical tests. However, lower expression levels in E. coli and their nature requiring two variable domains (VH and VL) for building the antigen-binding sites reduce their utility in genetic manipulation for generating improved species. Therefore, we attempted creation of new "antibody-like" proteins, which are composed of a single domain scaffold with much smaller molecular weight and can be expressed with a satisfactory yield in E. coli. We chose the biotin-binding protein, streptavidin monomer (stav'), as the basic scaffold. After several steps of mutagenesis and selection for their loop structures protruding from the scaffold, we succeeded in obtaining stav' mutants that had gained novel binding specificities targeting a steroid, estradiol.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Two chalcones, 4-hydroxyderricin and xanthoangelol, stimulate GLUT4-dependent glucose uptake through the LKB1/AMP-activated protein kinase signaling pathway in 3T3-L1 adipocytes2015
Author(s)
Ohta, Mitsuhiro; Fujinami, Aya; Kobayashi, Norihiro; Amano, Akiko; Ishigami, Akihito; Tokuda, Harukuni; Suzuki, Nobutaka; Ito, Fumitake; Mori, Taisuke; Sawada, Morio;
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Journal Title
Nutrition Research
Volume: 35(7)
Issue: 7
Pages: 618-625
DOI
Related Report
Peer Reviewed
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[Journal Article] Cellular Interaction and Cytotoxicity of the Iowa Mutation of Apolipoprotein A-I (ApoA-IIowa) Amyloid Mediated by Sulfate Moieties of Heparan Sulfate2015
Author(s)
Kuwabara, K., Nishitsuji, K., Uchimura, K., Hung, S.C., Mizuguchi, M., Nakajima, H., Mikawa, S., Kobayashi, N., Saito, H., and Sakashita, N.
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Journal Title
J Biol Chem
Volume: 290
Issue: 40
Pages: 24210-24221
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Book] 免疫測定法2014
Author(s)
生物化学的測定研究会編
Total Pages
323
Publisher
講談社
Related Report
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