| Project/Area Number |
25460710
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
Emi Mitsuru 兵庫医科大学, 医学部, 非常勤講師 (90221118)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAOKI TOMOKO 兵庫医科大学, 医学部, 名誉教授 (10172868)
吉川 良恵 兵庫医科大学, 医学部, 助教 (10566673)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIKAWA YOSHIE 兵庫医科大学, 医学部, 助教 (10566673)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 悪性中皮腫 / 腎細胞がん / 3p21 / BAP1 / biallelic deletion / ゲノム再構成 / SETD2 / ゲノム不安定性 / ゲノムコピー数 / 環境癌 / アレイCGH / MLPA |
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| Outline of Final Research Achievements |
In the present study, we sought to analyze environment-associated cancers, i.e., kidney cancer and malignant mesothelioma, for genome structural abnormalities with various advanced genome technologies, such as high-density CGH array, targeted NGS, MLPA, to search for copy number alterations in a chromosome region 3p21 that is suspected to be unstable with our previous knowledge. A simple contiguous large deletion of one allele (LOH) was observed as a characteristic event in kidney cancer. On the contrary, we detected multiple minute genome homozygous deletions in 20/33 tumors in malignant mesotheliomas. Those minute deletions were clustered in 47-48 Mb centered by SETD2 and 52-53 Mb region centered by BAP1. Those tumors presented with homozygous deletion of average of 4.7 genes. Therefore, unlike kidney cancer, key-events in mesothelioma development may associated with multiple minute copy number loss resulted by synchronous fragmentation of 3p21 and aberrant repair.
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