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Analysis of brain-enriched guanylate kinase-associated protein (BEGAIN) as a novel neuropathic pain-related protein in spinal lamina Iii

Research Project

Project/Area Number 25460729
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pain science
Research InstitutionKansai Medical University

Principal Investigator

KATANO Tayo  関西医科大学, 医学部, 講師 (60469244)

Co-Investigator(Kenkyū-buntansha) ITO Seiji  関西医科大学, 医学部, 教授 (80201325)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords神経障害性疼痛 / BEGAIN / プロテオミクス / 脊髄後角 / IIi層 / PSD / シナプス / 慢性疼痛 / 神経可塑性
Outline of Final Research Achievements

Maintenance of neuropathic pain caused by peripheral nerve injury critically depends on the phosphorylation of GluN2B, a subunit of NMDA receptor, at Tyr1472 (Y1472). Here we took an advantage of comparative proteomic analysis between wild-type and knockin mice with a mutation of Y1472 to Phe of GluN2B (Y1472F-KI) to search for PSD proteins in the spinal dorsal horn that mediate signaling of neuropathic pain downstream of phosphorylated Y1472 GluN2B. We identified brain-enriched guanylate kinase-associated protein (BEGAIN) as increased protein in wild-type, but not in Y1472F-KI mouse after peripheral nerve injury by proteomic analysis.
BEGAIN was localized in spinal lamina IIi. Moreover, neuropathic pain, but not physiological pain was significantly attenuated in the knockout mice of BEGAIN. These results indicate that BEGAIN was involved in pathological pain transmission through NMDAR activation following the phosphorylation of GluN2B at Y1472 in spinal lamina IIi.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Role of c-Jun N-terminal kinase in late nerve regeneration monitored by in vivo imaging of thy1-yellow fluorescent protein transgenic mice.2016

    • Author(s)
      Nguyen H. T., Katano, T., Matsumura, S., Pham M. V., Muratani, T., Minami, T. and Seiji Ito.
    • Journal Title

      Eur. J. Neurosci.

      Volume: 43 Issue: 4 Pages: 548-560

    • DOI

      10.1111/ejn.13139

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 脊髄に起因する痛み~脊髄の構造と慢性化をおこす中枢性感作~ Chronic pain is induced by central sensitization in spinal dorsal horn.2015

    • Author(s)
      片野泰代、伊藤誠二
    • Journal Title

      Practice of Pain Management

      Volume: 第18号, (Vol.6, No.1) Pages: 22-25

    • Related Report
      2014 Research-status Report
  • [Journal Article] Identification of nitrated tyrosine residues of protein kinase G-Ia by mass spectrometry.2014

    • Author(s)
      Lu J, Yao I, Shimojo M, Katano T, Uchida H, Setou M, Ito S
    • Journal Title

      Analytical and bioanalytical chemistry

      Volume: 406 Issue: 5 Pages: 1387-1396

    • DOI

      10.1007/s00216-013-7535-4

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of Nax sodium channel in peripheral nerve regeneration via lactate signaling.2013

    • Author(s)
      Unezaki, S., Katano, T., Hiyama, T.Y., Nguyen, H. T., Yoshii, S., Noda, M., and Ito, S.
    • Journal Title

      European Journal of Neuroscience

      Volume: 39 Issue: 5 Pages: 720-729

    • DOI

      10.1111/ejn.12436

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 脊髄後角における新規慢性疼痛関連分子の同定2015

    • Author(s)
      片野泰代、福田正史、山崎真耶、阿部学、渡辺雅彦、古江秀昌、矢尾育子、西田和彦、奥村宣明、中澤敬信、山本雅、崎村健司、高尾敏文、伊藤誠二
    • Organizer
      2015年度包括型脳科学研究推進支援ネットワーク 冬のシンポジウム
    • Place of Presentation
      一橋大学一橋講堂(東京・千代田区)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] New mechanisms for pain control and clinical potential.2015

    • Author(s)
      Nguyen H. Tu, Tayo Katano, Shinji Matsumura and Seiji Ito
    • Organizer
      International Conference on Pain-Anesthesiology and pain control
    • Place of Presentation
      Hanoi (Vietnam)
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of c-Jun N-terminal kinase in neurite extension of cultured DRG neurons.2015

    • Author(s)
      Nguyen, H. T., Matsumura, S. Katano, T., and Ito, S.
    • Organizer
      The 45th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Chicago (USA)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Localization of neuropathic pain-related protein, BEGAIN in the spinal dorsal horn.2015

    • Author(s)
      Katano, T., Watanabe, M., Yamazaki, M., Abe, M., Yao, I., Sakimura, K. and Ito, S.
    • Organizer
      The 45th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Chicago (USA)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two feasible mouse models for neuropathy.2015

    • Author(s)
      Pham, M.V., Nguyen, H.T., Katano, T., Matsumura, S. and Ito, S.
    • Organizer
      The 2nd Gene & Immunotherapy Conference in Vietnam
    • Place of Presentation
      Ho Chi Minh (Vietnam)
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of c-Jun N-terminal kinase in peripheral nerve regeneration.2014

    • Author(s)
      Nguyen, H. T., Katano, T., Matsumura, S. and Ito, S.
    • Organizer
      The 44th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C.
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Involvement of endothelin in peripheral nerve regeneration.2014

    • Author(s)
      Ito, S., Nguyen, H. T., Matsumura, S. and Katano, T.
    • Organizer
      The 44th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C.
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] CaMKIIキナーゼ不活型KIマウスにおける慢性疼痛モデル脊髄後角のCaMKIIのシナプス局在2014

    • Author(s)
      矢尾育子、松村伸治、片野泰代、山肩葉子、井本敬二、伊藤誠二
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 成熟した疼痛研究の新しい展開 New departure of mature pain research.2014

    • Author(s)
      伊藤誠二、片野泰代、松村伸治、西田和彦
    • Organizer
      日本ペインクリニック学会第48回大会
    • Place of Presentation
      新宿(京王プラザホテル)
    • Year and Date
      2014-07-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 末梢神経再生モデルを用いた神経再生におけるエンドセリンの関与 Involvement of endothelin in peripheral nerve regeneration using tubing and osmotic pump model.2014

    • Author(s)
      Nguyen Huu Tu、片野泰代、松村伸治、伊藤誠二
    • Organizer
      第36回日本疼痛学会
    • Place of Presentation
      大阪(KKRホテルオーサカ)
    • Year and Date
      2014-06-20 – 2014-06-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Involvement of neuropathic pain-related protein-B(NPRP-B), a novel functional molecule, in inflammatory and neuropathic pain in vivo.2013

    • Author(s)
      Katano, T., Yao, I., Yamazaki, M., Abe, M., Fukuda, M., Okumura, N., Takao, T., Sakimura, K. and Ito, S.
    • Organizer
      The 43rd annual meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, America
    • Related Report
      2013 Research-status Report
  • [Presentation] Proteomic approach of nitrated tyrosine residues of protein kinase G-Iα.2013

    • Author(s)
      Ito, S., Lu, J., Shimojo, M., Katano, T., Uchida, H. and Yao, I.
    • Organizer
      The 43rd annual meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, America
    • Related Report
      2013 Research-status Report
  • [Presentation] Energy coupling of axon-Schwann cells by Nax and endothelin in nerve regeneration2013

    • Author(s)
      Nguyen H. T., Katano, T., Hiyama, Y. T., Noda, M. and Ito, S.
    • Organizer
      The 44th NIPS International Symposium and The 5th Asian Pain Symposium
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎市)
    • Related Report
      2013 Research-status Report
  • [Book] Studies on Pediatric Disorders (Oxidative Stress in Applied Basic Research and Clinical Practice)2014

    • Author(s)
      Katano, T. Ito, S
    • Total Pages
      494
    • Publisher
      Human Press
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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