| Project/Area Number |
25460833
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene and public health
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
Takatori Satoshi 大阪府立公衆衛生研究所, その他部局等, 研究員 (90311480)
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| Co-Investigator(Kenkyū-buntansha) |
OKIHASHI Masahiro 大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (60250312)
KITAGAWA Yoko 大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (20280836)
FUKUI Naoki 大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (90516717)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUTA Masakazu 大阪府立大学, 地域連携研究機構・放射線研究センター, 教授 (40181458)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 照射食品 / 検知法 / 5,6-dihydrothymidine / LC-MS/MS / 生レバー / 食の安全 / 放射線照射 |
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| Outline of Final Research Achievements |
A method for detecting irradiation histories of foods including raw beef livers and shrimps was developed by measuring 5,6-dihydrothymidine (dDHT) using LC-MS/MS. Liver DNA was extracted using phenol-chloroform extraction followed by precipitation in 50% ethanol. DNA was then enzymatically digested and nucleosides were purified using an OASIS MCX column. dDHT and thymidine (Thd) contents of resulting test solutions were analyzed using LC-MS/MS. dDHT was detected specifically after gamma-irradiation. Concentration ratios of dDHT to Thd in the test solutions increased dose-dependently after irradiation at 1.0-11.3 kGy, which included the practical dose for sterilization of 2-7 kGy. Thus, this method is a candidate for the detection of irradiation histories of foods from which DNA can be extracted.
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