Application of HRM analysis to multiplex genotyping of useful SNPs for forensic investigations

Research Project

Project/Area Number	25460868
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Legal medicine
Research Institution	Tottori University
Principal Investigator	NAKAGAWA MAYUMI 鳥取大学, 医学部, 講師 (00243410)
Co-Investigator(Renkei-kenkyūsha)	YUASA Isao 鳥取大学, 医学部, 准教授 (00093633)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	HRM解析 / SNP / マルチプレックス解析
Outline of Final Research Achievements	This study was performed to application of HRM analysis to genotyping of useful SNP for forensic investigations. We developed the procedure to determining the genotypes of c.C1423T of the ADAMTS13 gene by HRM analysis. And we have established a multiplex genotyping method for c.G261del and c.G803C of the ABO gene in a single reaction. The HRM analysis to genotyping of useful SNP for forensic investigations is an accurate and conventional method.