| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
C57BL/6j and apolipoprotein E and low-density lipoprotein receptor double knockout male mice were randomly divided into alcoholic or chronic mental stress (CMS) study, and then reared for 16 weeks. Ethanol promoted the development of atherosclerosis and induced the down-regulation of endothelial cell layer repair gene (CD34) and up-regulations of the monocyte chemotactic protein-1 and matrix metallopeptidase 9 (an enzyme involved in the extracellular matrix degradation) gene expressions in abdominal aortas. CMS, especially in mice with hyperlipidemia, showed the high risk of cardiovascular events via the developed atherosclerosis accompanied the down-regulation of nitric oxide synthase 3 and up-regulation of lectin-like oxidized low-density lipoprotein receptor-1 gene expressions (the genes that related to the endothelial dysfunction).
The chronic heavy drink and CMS could be the risk factors for cardiovascular disease, especially in subject with hyperlipidemia.
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