Detection of infectious microbes by the Genome Profiling method

Research Project

Project/Area Number	25460875
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Legal medicine
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Ikegaya Hiroshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (30292874)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	ゲノムプロファイリング / JCウイルス / BKウイルス / 17SrDNA / 溺死 / JC virus / BK virus / ウイルス / 微生物 / 16SrDNA
Outline of Final Research Achievements	Detection of two viral species were successful by the Genome profiling(GP) method using random PCR. Prior to the cluster analysis, viral species were detectable by the Species Identification dots (Spiddos). Those viruses were detectable from human urine samples. Various bacteria species were detectable from the postmortem human blood samples by the GP method using 17SrDNA amplification. The diagnosis of drawing were successful by the GP method using 17SrDNA amplification.

Report

(4 results)