| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
I have developed a fully validated method for the analysis of TTX in serum and urine. In this method, serum and urine samples were extracted using MonoSpin CBA or amide columns, followed by LC-MSMS analysis. Then, I have developed and validated a rapid determination of aconitine, hypaconitine, jesaconitine, mesaconitine, and colchicine in serum, urine, and plants using LC-MSMS. All targeted compounds were extracted by C18 monolithic spin column. Hypholoma sp.The toxic components of Hypholoma and O. japonicas are fasciculole E and F and illudin S, respectively. I have developed a method for rapid detection of fasciculol E and F and illusion S in mushrooms using LC-MSMS. Myristicin is the main component of nutmeg essential oil. A GC-MS method was developed and validated for serum for the determination of myristicin. A GC-MS method was more sensitive 100 times than LC-MSMS. The application of all methods were further verified by analysis of biological materials from a poisoning patients.
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