Forensic diagnosis for heatstroke-a search for useful biomarkers by metabolic profiling

• Project/Area Number: 25460880
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Osaka Medical College
• Principal Investigator: Suzuki Koichi 大阪医科大学, 医学部, 教授 (60171211)
• Co-Investigator(Kenkyū-buntansha): SATO Takako 大阪医科大学, 医学部, 准教授 (10530420) ; TSUCHIHASHI Hitoshi 名古屋大学, 医学系研究科, 招聘教員 (40596029)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: メタボロミクス / 熱中症 / 死後経過時間 / ラット

Outline of Final Research Achievements

To search for biomarkers of heatstroke, we demonstrated by GC-MS/MS based plasma metabolic profiling that various endogenous metabolites changed with elevated body temperatures in heat-stressed animal models. We detected 72 metabolites, of which 18 metabolites (9 amino acids, 6 organic acids, and 3 monosaccharides) were found to be statistically correlated with rised body temperatures. These metabolites had a relationship with the energy productions. In addition, in order to understand the effect of postmortem interval (PMI) on those metabolites in plasma, we investigated the change of metabolites with time after death towards the future forensic casework. As a result, we constructed a PLS regression model which could successfully predict each PMI.

Research Products

• [Journal Article] A preliminary study on postmortem interval estimation of suffocating rats by GC-MS/MS-based plasma metabolic profiling (2015)
  • Author(s): T Sato, K Zaitsu, K Tsuboi, M Nomura, M Kusano, N Shima, S Abe, A Ishii, H Tsuchihashi, K Suzuki
  • Journal Title: Analytical Bioanalytical Chemistry Volume: in press Issue: 13 Pages: 3659-3665
  • DOI: 10.1007/s00216-015-8584-7
  • Peer Reviewed
• [Presentation] ラット熱中症モデルにおけるメタボロミクス解析 (2015)
  • Author(s): 佐藤貴子、財津 桂、坪井健人、野村和克、岩田美佐、志摩典明、阿部俊太郎、石井晃、土橋 均、鈴木廣一
  • Organizer: 第99次日本法医学会学術全国集会
  • Place of Presentation: 高知市文化プラザかるぽーと
  • Year and Date: 2015-06-10 – 2015-06-12
• [Presentation] Postmortem interval estimation of suffocated rats by plasma metabolic profiling (2014)
  • Author(s): Sato T, Zaitsu K, Tsuboi K, Nomura M, Shima N, Iwata M, Abe S, Ishii A, Tsuchihashi H, Suzuki K
  • Organizer: the 9th International Symposium on Advances in Legal Medicine
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2014-06-16 – 2014-06-20