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Rikkunshito increased hypothalamic SIRT1 activity

Research Project

Project/Area Number 25460922
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionHokkaido University

Principal Investigator

TAKEDA Hiroshi  北海道大学, 薬学研究科(研究院), 教授 (60261294)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords六君子湯 / 食欲
Outline of Final Research Achievements

To examine the roles of SIRT1 and AMPK in the orexigenic effect of rikkunshito, a ghrelin signaling potentiator, in vitro experiments using gherkin receptor(GHSR1a)-expressing HEK293 cells and human umbilical vein endothelial cells (HUVEC) were done. The elevated SIRT1 activity and protein expression through cAMP-CREB pathway was observed after ghrelin and rikkunshito treatment in these cells. Rikkunshito increased hypothalamic SIRT1 activity and SIRT1 protein expression of the heart in ICR mouse. Furthermore, rikkunshito prolonged survival in aged ICR mice. The potentiation of ghrelin signaling by rikkunshito not only acts as appetite stimulator but may provide a novel approach for the improvement of both health- and life-span in aging societies.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (27 results)

All 2016 2015 2014 2013 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Increased ghrelin signaling prolongs survival in mouse models of human aging through activation of sirtuin1.2016

    • Author(s)
      Fujitsuka N, Asakawa A, Morinaga A, Amitani MS, Amitani H, Katsuura G, Sawada Y, Sudo Y, Uezono Y, Mochiki E, Sakata I, Sakai T, Hanazaki K, Yada T, Yakabi K, Sakuma E, Ueki T, Niijima A, Nakagawa K, Okubo N, Takeda H, Asaka M, Inui A.
    • Journal Title

      Mol Psychiatry

      Volume: 21 Issue: 11 Pages: 1-11

    • DOI

      10.1038/mp.2015.220

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serotonin 2C receptor contributes to gender differences in stress-induced hypophagia in aged mice.2015

    • Author(s)
      Yamada C, Sadakane C, Nahata C, Saegusa Y, Nakagawa K, Okubo N, Ohnishi S, Hattori T, Takeda H
    • Journal Title

      Psychoneuroendocrinology

      Volume: 55 Pages: 81-93

    • DOI

      10.1016/j.psyneuen.2015.02.006

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gender Differences on Feeding Behavior and Ghrelin-Related Factors during Social Isolation in Mice.2015

    • Author(s)
      Yamada C, Saegusa Y, Nahata M, Sadakane C, Hattori T, Takeda H.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 10 Pages: e0140094-e0140094

    • DOI

      10.1371/journal.pone.0140094

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Herbal medicines for the treatment of cancer chemotherapy-induced side effects.2015

    • Author(s)
      Ohnishi S, Takeda H.
    • Journal Title

      Front Pharmacol

      Volume: 6 Pages: 14-14

    • DOI

      10.3389/fphar.2015.00014

    • NAID

      120005773990

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A New Strategy Using Rikkunshito to Treat Anorexia and Gastrointestinal Dysfunction.2015

    • Author(s)
      Saegusa Y, Hattori T, Nahata M, Yamada C, Takeda H.
    • Journal Title

      Evid Based Complement Alternat Med.

      Volume: 2015 Pages: 364260-364260

    • DOI

      10.1155/2015/364260

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Administration of exogenous acylated ghrelin or rikkunshito, an endogenous ghrelin enhancer, improves the decrease in postprandial gastric motility in an acute restraint stress mouse model.2014

    • Author(s)
      Nahata M, Saegusa Y, Sadakane C, Yamada C, Nakagawa K, Okubo N, Ohnishi S, Hattori T, Sakamoto N, Takeda H.
    • Journal Title

      Neurogastroenterol Motil

      Volume: 26 Issue: 6 Pages: 821-831

    • DOI

      10.1111/nmo.12336

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical characteristics of elderly patients with proton pump inhibitor-refractory non-erosive reflux disease from the G-PRIDE study who responded to rikkunshito.2014

    • Author(s)
      Sakata Y, Tominaga K, Kato M, Takeda H, Shimoyama Y, Takeuchi T, Iwakiri R, Furuta K, Sakurai K, Odaka T, Kusunoki H, Nagahara A, Iwakiri K, Furuta T, Murakami K, Miwa H, Kinoshita Y, Haruma K, Takahashi S, Watanabe S, Higuchi K, Fujimoto K, Kusano M, Arakawa T; G-PRIDE study group.
    • Journal Title

      BMC Gastroenterol

      Volume: 14 Issue: 1 Pages: 116-116

    • DOI

      10.1186/1471-230x-14-116

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of Rikkunshito (traditional Japanese medicine) on enteral feeding and the plasma ghrelin level in critically ill patients: a pilot study.2014

    • Author(s)
      Hayakawa M, 他6名
    • Journal Title

      Journal of Intensive Care

      Volume: 2 Issue: 1 Pages: 53-53

    • DOI

      10.1186/s40560-014-0053-4

    • NAID

      120005595707

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serotonin 2C receptor antagonism ameliorates novelty-induced hypophagia in aged mice.2013

    • Author(s)
      Nahata M, Muto S, Nakagawa K, Ohnishi S, Sadakane C, Saegusa Y, Iizuka S, Hattori T, Asaka M, Takeda H.
    • Journal Title

      Psychoneuroendocrinology.

      Volume: 38 Issue: 10 Pages: 2051-2064

    • DOI

      10.1016/j.psyneuen.2013.03.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pathophysiological basis of anorexia.2013

    • Author(s)
      Takeda H, Muto S, Nakagawa K, Ohnishi S, Sadakane C, Saegusa Y, Nahata M, Hattori T, Asaka M.
    • Journal Title

      Biol. Pharm. Bull.

      Volume: 36 Pages: 1401-1405

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cisplatin-induced anorexia and ghrelin.2013

    • Author(s)
      Hattori T, Yakabi K, Takeda H.
    • Journal Title

      Vitam Horm.

      Volume: 92 Pages: 301-317

    • DOI

      10.1016/b978-0-12-410473-0.00012-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Different response to chronic isolation stress between young and aged mice.2015

    • Author(s)
      Takeda H, Ohnishi S, Nakagawa K, Okudo N, Yamada C, Saegusa Y, Nahata M, Hattori T, Sakamoto, N
    • Organizer
      Digestive Disease Week 2015
    • Place of Presentation
      ワシントンDC(米国)
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The hypophagia after psychological stress in aged male mice is mediated by an interaction of activation of estrogen receptor α with serotonin 2C receptor.2015

    • Author(s)
      Ohnishi S, Nakagawa K, Okudo N, Yamada C, Saegusa Y, Nahata M, Sadakane C, Hattori T, Sakamoto, N, Takeda H
    • Organizer
      Digestive Disease Week 2015
    • Place of Presentation
      ワシントンDC(米国)
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] セロトニンによるグレリン分泌の調節機構2015

    • Author(s)
      武藤修一、大西俊介、武田宏司
    • Organizer
      第101回日本消化器病学会総会
    • Place of Presentation
      仙台国際センター (宮城県仙台市)
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ストレスによる老齢動物の摂食低下には性差が存在する2015

    • Author(s)
      大西俊介、武藤修一、武田宏司
    • Organizer
      第101回日本消化器病学会総会
    • Place of Presentation
      仙台国際センター (宮城県仙台市)
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] グレリンシグナルからみた六君子湯の作用メカニズム(漢方セミナー)2014

    • Author(s)
      武田 宏司
    • Organizer
      第56回日本平滑筋学会総会
    • Place of Presentation
      新横浜プリンスホテル(横浜)
    • Year and Date
      2014-08-08
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 栄養応答シグナルの解明と疾患治療への応用 グレリンシグナルおよびセロトニン2C受容体の意義2014

    • Author(s)
      武田宏司, 武藤 修一, 大西 俊介
    • Organizer
      第14回日本抗高齢医学会総会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2014-06-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] The Possibility of Rikkunshito As a Treatment for Anorexia: a Proposal From a Basic Research Perspective.2014

    • Author(s)
      Takeda H, Ohnishi S, Muto S, Nakagawa K, Yamada C, Saegusa Y, Nahata M, Sadakane C, Hattori T, Sakamoto, N.
    • Organizer
      Digestive Disease Week 2014
    • Place of Presentation
      シカゴ(米国イリノイ州)
    • Year and Date
      2014-05-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Rikkunshito, a Traditional Japanese Kampo Medicine, Ameliorates the Decrease in Feeding Behavior in Aged Mice Exposed to Stress via Enhancement of Endogenous Ghrelin Production.2014

    • Author(s)
      Muto S, Ohnishi S, Nakagawa K, Yamada C, Saegusa Y, Nahata M, Sadakane C, Hattori T, Sakamoto, N, Takeda H.
    • Organizer
      Digestive Disease Week 2014
    • Place of Presentation
      シカゴ(米国イリノイ州)
    • Year and Date
      2014-05-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Association of abnormal ghrelin dynamics with delayed gastric emptying in acute restraint-stressed mice.2014

    • Author(s)
      Takeda H, Ohnishi S, Muto S, Nakagawa K, Yamada C, Saegusa Y, Nahata M, Sadakane C, Hattori T, Sakamoto, N.
    • Organizer
      Digestive Disease Week 2014
    • Place of Presentation
      シカゴ(米国イリノイ州)
    • Year and Date
      2014-05-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Gender Difference Influences Food Intake Under an Emotional Stress via 5-HT2CR Activation in Aged Mice.2014

    • Author(s)
      Ohnishi S, Muto S, Nakagawa K, Yamada C, Saegusa Y, Nahata M, Sadakane C, Hattori T, Sakamoto, N Takeda H
    • Organizer
      Digestive Disease Week 2014
    • Place of Presentation
      シカゴ(米国イリノイ州)
    • Year and Date
      2014-05-04
    • Related Report
      2014 Research-status Report
  • [Presentation] ストレスによる胃機能異常にはグレリンシグナルの異常が関与する(ワークショップ)2014

    • Author(s)
      武藤 修一, 大西 俊介, 武田 宏司
    • Organizer
      第100回日本消化器病学会総会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Year and Date
      2014-04-24
    • Related Report
      2014 Research-status Report
  • [Presentation] ctivation of Corticotropin-Releasing Factor 2 and Melanocortin-4 Receptors Mediates the Persistent Loss of Appetite Induced by Stress Loading in Advanced Age Mice.

    • Author(s)
      Takeda H, Muto S, Ohnishi S, Nakagawa K, Sadakane C, Saegusa Y, Nahata M, Yamada C, Hattori T, Asaka M. A
    • Organizer
      Digestive Disease Week 2013
    • Place of Presentation
      オーランド(米国フロリダ州)
    • Related Report
      2013 Research-status Report
  • [Presentation] Serotonin 2C Receptor Antagonism Improves Stress-Induced Anorexia in Aged Mice.

    • Author(s)
      2) Muto S, Ohnishi S, Nakagawa K, Sadakane C, Saegusa Y, Nahata M, Yamada C, Hattori T, Asaka M, Takeda H.
    • Organizer
      Digestive Disease Week 2013
    • Place of Presentation
      オーランド(米国フロリダ州)
    • Related Report
      2013 Research-status Report
  • [Presentation] Involvement of Ghrelin Signaling Dysfunction in Acute Restraint Stress-Induced Delayed Gastric Emptying.

    • Author(s)
      3) Ohnishi S, Muto S, Nakagawa K, Sadakane C, Saegusa Y, Nahata M, Yamada C, Hattori T, Asaka M, Sakamoto, N Takeda H.
    • Organizer
      Digestive Disease Week 2013
    • Place of Presentation
      オーランド(米国フロリダ州)
    • Related Report
      2013 Research-status Report
  • [Presentation] 急性拘束ストレスによる胃排出遅延にはグレリンシグナル伝達障害が関与する

    • Author(s)
      武田宏司
    • Organizer
      第55回日本平滑筋学会総会
    • Place of Presentation
      旭川市大雪クリスタルホール(北海道旭川市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ストレスによる老齢動物の摂食低下には5-HT2C受容体の機能亢進が関与する

    • Author(s)
      大西俊介、武藤修一、武田宏司
    • Organizer
      JDDW2013 (シンポジウム)
    • Place of Presentation
      グランドプリンスホテル新高輪他(東京都)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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