Innate immunity-mediated regulation on the induction of precancerous lesions in the stomach.

• Project/Area Number: 25460925
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: Asano Naoki 東北大学, 大学病院, 助教 (20526454)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: NOD1 / Helicobacter pylori / 腸上皮化生 / 胃粘膜萎縮 / Sox2

Outline of Final Research Achievements

Innate immunity-related molecule NOD1 suppressed Helicobacter pylori (H. pylori) infection induced Cdx2 and Muc2 induction and NF-κB p65 activation in gastric epithelial cells. On the other hand, NOD1 was required for the maintenance of ATP4a expression. The changes in gene expression upon H. pylori infection were observed as induction of intestinal metaplasia and gastric mucosal atrophy in the stomachs of H. pylori-infected NOD1-deficient mice 12 months after infection. The expression of TRAF3 was lower in these mice compared to NOD1-intact mice and this could be one of the causes for the induction of precancerous lesions in H. pylori-infected stomach.

Research Products

• [Int'l Joint Research] National Institutes of Health(米国)
• [Journal Article] Cdx2 Expression and Intestinal Metaplasia Induced by H. pylori Infection of Gastric Cells Is Regulated by NOD1-Mediated Innate Immune Responses (2016)
  • Author(s): Asano N, Imatani A, Watanabe T, Fushiya J, Kondo Y, Jin X, Ara N, Uno K, Iijima K, Koike T, Strober W, Shimosegawa T
  • Journal Title: Cancer Research Volume: 76 Issue: 5 Pages: 1135-1145
  • DOI: 10.1158/0008-5472.can-15-2272
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] NOD1 Expression in Gastric Epithelial Cells Is Down-Regulated By Helicobacter pylori Infection Through the Methylation of Its CpG Island By DNA Methyltransferases (2016)
  • Author(s): Mikami T, Asano N, Imatani A, Jin X, Kondo Y, Hatta W, Ara N, Asanuma K, Koike T, Shimosegawa T
  • Organizer: Digestive Disease Week 2016
  • Place of Presentation: San Diego, CA, U.S.A.
  • Year and Date: 2016-05-21
  • Int'l Joint Research