Functional analysis of large intergenic non-coding RNAs regulated by p53 in cancers of digestive organs
| Project/Area Number |
25460929
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | p53 / lncRNA / ChIP-seq / 癌 / lincRNA / non-coding RNA / 転写 / アポトーシス |
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| Outline of Final Research Achievements |
p53 is one of the most important known tumor suppressor genes, and it is inactivated in approximately half of human cancers. To identify the direct transcriptional targets of p53, we performed chromatin immunoprecipitation together with next-generation sequencing (ChIP-seq) and searched for p53 binding motifs across the entire human genome. Among the identified ChIP-seq peaks, approximately half were located in an intergenic region. Therefore, we assumed large intergenic non-coding RNAs (lincRNAs) to be major targets of the p53 family. Through a combination of ChIP-seq and in silico analyses, we found 23 lincRNAs that are upregulated by the p53 family. Additionally, knockdown of specific lincRNAs modulated p53-induced apoptosis and promoted the transcription of a gene cluster. Our results suggest that p53 family members and lincRNAs constitute a complex transcriptional network involved in various biological functions and tumor suppression.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Contextual niche signals towards colorectal tumor progression by mesenchymal stem cell in the mouse xenograft model.2015
Author(s)
Nakagaki S, Arimura Y, Nagaishi K, Isshiki H, Nasuno M, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y.
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Journal Title
Journal of Gastoroenterology
Volume: 50
Issue: 9
Pages: 962-74
DOI
Related Report
Peer Reviewed
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[Journal Article] Mesenchymal stem cells cancel azoxymethane-induced tumor initiation2014
Author(s)
Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y
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Journal Title
Stem Cells
Volume: 32
Issue: 4
Pages: 913-925
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Conditioned mesenchymal stem cells produce pleiotropic gut trophic factors2014
Author(s)
Watanabe S, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nasuno M, Yamashita K, Idogawa M, Naishiro Y, Murata M, Adachi Y, Fujimiya M, Imai K, Shinomura Y
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Journal Title
J Gastroenterol
Volume: 49
Issue: 2
Pages: 270-282
DOI
Related Report
Peer Reviewed
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