| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Mouse gastric mucins (MGM) were prepared from corpus and antral mucosa of C57BL/6 mouse stomach. Mucin-type O-glycans were prepared and analyzed using two methods. Firstly, the reduced-form O-glycans were obtained from MGM by alkaline-borohydride treatment and after permethylation analyzed by MALDI-TOF/MS and MS/MS. Secondly, the O-glycans bearing reducing terminal GalNAc as an intact form were obtained from MGM by treatment with anhydrous hydrazine and, after derivatized with anthranilic acid, they were separated by hydrophilic interaction liquid chromatography (HILIC) and fractionated. Each O-glycan was characterized by MALDI-TOF/MS and MS/MS. In the latter methods, it was found that malonic acid suppressed O-glycan degradation during hydrazine treatment, and the method was optimized on the reaction conditions.
Both methods showed that corpus and antral mucins displayed similar glycosylation patterns, but several glycans, especially acidic ones, were characteristic to each mucin.
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