Detection of Early Gastric Cancer with DNA Methylation Analysis of Gastric Washes

• Project/Area Number: 25460937
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Watanabe Yoshiyuki 聖マリアンナ医科大学, 医学部, 講師 (90329243)
• Co-Investigator(Kenkyū-buntansha): Maehata Tadateru 慶應義塾大学, 医学部, 助教 (90534199)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 胃がん / 胃洗浄廃液 / 分子マーカー / DNAメチル化 / ピロリ菌 / 分子腫瘍マーカー / メチル化

Outline of Final Research Achievements

Although minimal invasive treatment is widely accepted for early stage of gastric cancer (EGC), we still do not have any appropriate risk markers to detect residual neoplasia and some potential of recurrence. Our goal is to verify the potency of our three selected candidate genes (Sox17, MINT25, miR34) as a treatment marker for EGC. 352 EGC cases were registered and endoscopically removed. 182 cases could followed endoscopically for 2 years (51.7%). Sensitivity, specificity and AUC were as follows. Sox17 (70.0%, 48.1%, 0.646), MINT25 (70.0%, 35.2% 0.555), miR34 (65.0%, 49.4%, 0.578). We have developed a new methodology for gastric cancer detection by DNA methylation in gastric washes. It is easy and useful for early detection of recurrence after endoscopic treatment in GCa patients.

Research Products

• [Journal Article] Analysis of Helicobacter pylori genotypes in clinical gastric wash samples. (2016)
  • Author(s): Miyamoto S, Watanabe Y, Oikawa R, Ono S, Mabe K, Kudo T, Yamamoto H, Itoh F, Kato M, Sakamoto N.
  • Journal Title: Tumour Biology Volume: Jan 29. Issue: 8 Pages: 1-10
  • DOI: 10.1007/s13277-016-4886-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] DNA methylation at hepatitis B viral integrants is associated with methylation at flanking human genomic sequences. (2015)
  • Author(s): Watanabe Y, Yamamoto H, Oikawa R, Toyota M, Yamamoto M, Kokudo N, Tanaka S, Arii S, Yotsuyanagi H, Koike K, Itoh F.
  • Journal Title: Genome Research Volume: 25(3) Issue: 3 Pages: 328-337
  • DOI: 10.1101/gr.175240.114
  • Peer Reviewed / Open Access
• [Journal Article] An updated review of gastric cancer in the next-generation sequencing era : Insights from bench to bedside and vice versa (2014)
  • Author(s): Yamamoto H (他14名), Nosho K, Shinomura Y
  • Journal Title: World J Gastroenterol Volume: 20 Issue: 14 Pages: 3927-3937
  • DOI: 10.3748/wjg.v20.i14.3927
  • Peer Reviewed / Open Access
• [Presentation] The BARHL2 Gene Is Silenced by Promoter Hypermethylation in Early Stage of Gastric Cancer (2015)
  • Author(s): Yoshiyuki Watanabe, Ritsuko Oikawa, Hiroyuki Yamamoto, Fumio Itoh
  • Organizer: Digestive Disease Week (DDW) 2015
  • Place of Presentation: San Diego Convention Center, San Diego CA, USA
  • Year and Date: 2015-05-18
  • Int'l Joint Research
• [Presentation] Hypermethylation of BARHL2 gene is useful as a molecular marker in early stage of gastric cancer (2015)
  • Author(s): Yoshiyuki Watanabe, Ritsuko Oikawa, Hiroyuki Yamamoto, Fumio Itoh
  • Organizer: American Association for Cancer Research (AACR) 2015
  • Place of Presentation: Ernest N. Morial Convention Center, ew Orleans, LO, USA
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] CYP26C1 gene is highly methylated and correlated with chemoradiation therapy in esophagus squamous cell carcinoma (2014)
  • Author(s): Yoshiyuki Watanabe, Ritsuko Oikawa, Yoshihito Yoshida, Hiroyuki Yamamoto, Itoh, Fumio
  • Organizer: Digestive Disease Week 2014
  • Place of Presentation: Chicago, IL, US
  • Year and Date: 2014-05-03
• [Presentation] CYP26C1 gene is highly methylated and correlated with chemoradiation therapy in esophagus squamous cell carcinoma (2014)
  • Author(s): Yoshiyuki Watanabe, Ritsuko Oikawa, Yoshihito Yoshida, Hiroyuki Yamamoto, Itoh, Fumio
  • Organizer: American Association for Cancer Research Annual Meeting 2014
  • Place of Presentation: San Diego Convention Center, San Diego, CA, US
  • Year and Date: 2014-04-06
• [Patent(Industrial Property Rights)] 胃粘膜洗浄液を利用した疾患関連マーカー検出法 (2013)
  • Inventor(s): 渡邊嘉行
  • Industrial Property Rights Holder: 渡邊嘉行
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-04-16
  • Acquisition Date: 2014-05-15