| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Duodenal epithelial cells expressed claudin (CLDN) 1, CLDN2, CLDN3, CLDN4, CLDN7，CLDN8, CLDN15, CLDN18, JAM-A, Nectin-1, Nectin-3, ZO-1, and ZO-3. The expression of each protein was different in FD patients and CLDN8 seemed to decrease in FD patients.
In vitro, IFNg, but not TNFa or IL-1b, significantly increased IL-33 mRNA compared with untreated cells. The combination of deoxycholic acid (DCA) and IFNg significantly up-regulated IL-33 mRNA compared with IFNg alone, and significantly induced more IL-8 and IL-6 compared with DCA or IFNg alone. DCA and IFNg-induced IL-33 precedes the up-regulation of IL-8 and IL-6. In order to investigate the function of nuclear up-regulated IL-33, the expression was suppressed with siRNA. IL-33 knockdown dampened IFNg-induced IL-8 and IL-6 production. These data suggest that nuclear IL-33 exaggerates the production of inflammatory cytokines.
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