| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Ncx-deficient (KO) mice exhibited increased number of enteric neuron (EN) and hyper sensitive to DSS induced colitis model. In this study, we characterized the fecal microbiota to examine whether increased number of EN affects enteric flora. CFUs of aerobic bacteria increased in the KO mice compared to those of wild type mice. Meta-genome analysis revealed that the family Enterobacteriaceae were significantly higher in the KO mice compared with the wild type mice. Since bacterial NO reductase gene V (NorV) which detoxificate NO, determine a virulence of bacteria, more NorV+ bacteria were detected from the feces of the KO mice compared to that of wild type mice. To elucidate whether the dysbiosis affect the severity of the DSS-induced colitis, fecal transfer experiments were carried out. Mice transferred with feces of the KO mice developed more severe colitis than wild type transferred one. Our study implies that EN participates in regulation of intestinal microbiota.
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