Identification of the amino acids regulating barrier function of the intestinal mucosal layers
Project/Area Number |
25460943
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
|
Research Institution | Chiba University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Tomoo 千葉大学, 医学部附属病院, 助教 (40396677)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 腸管粘膜上皮 / バリア機能回復 / アミノ酸 / グリシン / グルタミン / TNFα / クローン病 / 経腸栄養剤 / バリア機能 / TNF-α / glycine / glutamine / claudin-14 / TER / claudin-2 / IL-6 / IL-10 |
Outline of Final Research Achievements |
We first carried out a retrospective cohort study for 74 patients with Crohn's disease (CD) treated with infliximab (IFX). The longest observation period was 12 years. This study demonstrated that concomitant use of enteral nutrient (EN) at a dose of 600 kcal/day yielded a sustained response to IFX maintenance therapy in patients with CD. We next investigated whether the amino acids included in the EN can affect the barrier function of the large intestinal mucosal epithelial cell layers. We found that glycine and glutamine individually recovered barrier function of the epithelial cell layers treated with TNFα, a well-known cytokine to decrease the barrier function. The results of PCR array showed that the treatment with glycine significantly increased the gene expression of claudin-14, suggesting that up-regulation of claudin-14 is one of the mechanism of EN.
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Report
(4 results)
Research Products
(4 results)