| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
The presence of ingested fat in the proximal small intestine induces secretion of CCK from a type of enteroendocrine cells: I-cells. It is now clear that GPR40 is the functional sensor of FFAs of CCK cells (I cells). However, we still need to clarify (1) the precise structural requirements (size of aggregates or micelles, etc.) of FFAs as effective ligands of GPR40 and (2) the roles of gut luminal environment for proper fatty-acid sensing. In this study, to clarify the cellular mechanisms by which bile acids and gut luminal environment influence fatty-acid sensing mechanisms, we examined the combined effects of fatty acids and bile acids on [Ca2+]i in PC12 cells expressing GPR40.
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