Role of Hsp27 and SPARC in the carcinogenesis of colorectal cancer

• Project/Area Number: 25460958
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Naito Yuji 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00305575)
• Co-Investigator(Kenkyū-buntansha): UCHIYAMA Kazuhiko 京都府立医科大学, 医学(系)研究科(研究院), 助教 (50298428) ; TAKAGI Tomohisa 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70405257) ; ITO Tomoko 名古屋大学, 医学(系)研究科(研究院), 助教 (80329648)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 翻訳後修飾熱ショック蛋白質 / SPARC / バイオマーカー / 腸管粘膜免疫 / 大腸癌 / メチルグリオキザール / 熱ショックタンパク質

Outline of Final Research Achievements

The present study has demonstrated that 1) methylglyoxal-modified Hsp27 protein (MG-Hsp27) and secreted protein acidic and rich in cysteine (SPARC) secreted from muscle play a crucial role in the carcinogenesis of colorectal cancer, 2) SPARC protein could reduce intestinal inflammation via the inhibition of inflammatory cytokines and activated T lymphocytes, and 3) serum levels of MG-HSP and SPARC did not changes in the clinical stages of colorectal cancers.

Research Products

• [Journal Article] Rapamycin Improves Mortality Following Intestinal Ischemia-Reperfusion via the Inhibition of Remote Lung Inflammation in Mice. Digestion. 2015;92:211-9. (2015)
  • Author(s): Iida T, Takagi T, Katada K, Mizushima K, Fukuda W, Kamada K, Uchiyama K, Handa O, Ichikawa H, Naito Y, Itoh Y.
  • Journal Title: Digestion Volume: 92 Issue: 4 Pages: 211-219
  • DOI: 10.1159/000439300
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Peptidomic analysis via one-step direct transfer technology for colorectal cancer biomarker discovery (2015)
  • Author(s): Uchiyama K, Naito Y, Yagi N, Mizushima K, Higashimura Y, Hirai Y, Okayama T, Yoshida N, Katada K, Kamada K, Handa O, Ishikawa T, Takagi T, Konishi H, Nonaka D, Asada K, Tanaka K, Nakanishi M, Otsuji E, Itoh Y.
  • Journal Title: J Proteomics Bioinform Volume: S5 Pages: 1-8
  • DOI: 10.4172/jpb.s5-005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The therapeutic potential of carbon monoxide for inflammatory bowel disease (2015)
  • Author(s): Takagi T, Uchiyama K, Naito Y.
  • Journal Title: Digestion Volume: 91(1) Issue: 1 Pages: 13-18
  • DOI: 10.1159/000368765
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Propionate Promotes Fatty Acid Oxidation through the Up-Regulation of Peroxisome Proliferator-Activated Receptor α in Intestinal Epithelial Cells (2015)
  • Author(s): Higashimura, Y., Naito Y., Takagi T., Uchiyama K., Mizushima K., and Yoshikawa T.
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 61 Issue: 6 Pages: 511-515
  • DOI: 10.3177/jnsv.61.511
  • NAID: 130005125490
  • ISSN: 0301-4800, 1881-7742
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] New genetic biomarkers predicting azathioprine blood concentrations in combination therapy with 5-aminosalicylic Acid. (2014)
  • Author(s): Uchiyama K, Takagi T, Iwamoto Y, Kondo N, Okayama T, Yoshida N, Kamada K, Katada K, Handa O, Ishikawa T, Yasuda H, Sakagami J, Konishi H, Yagi N, Naito Y, Itoh Y
  • Journal Title: PLoS One Volume: 24;9(4) Issue: 4 Pages: e95080-e95080
  • DOI: 10.1371/journal.pone.0095080
  • Peer Reviewed / Open Access
• [Journal Article] Heme oxygenase-1 and anti-inflammatory M2 macrophages (2014)
  • Author(s): Naito, Y. Takagi, T. Higashimura, Y
  • Journal Title: Arch Biochem Biophys Volume: 564 Pages: 83-86
  • DOI: 10.1016/j.abb.2014.09.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lipid hydroperoxide-derived modification of proteins in gastrointestinal tract (2014)
  • Author(s): Naito, Y. Takagi, T. Handa, O. Yoshikawa, T.
  • Journal Title: Subcell Biochem Volume: 77 Pages: 137-148
  • DOI: 10.1007/978-94-007-7920-4_12
  • ISBN: 9789400779198, 9789400779204
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Preventive effect of agaro-oligosaccharides on non-steroidal anti-inflammatory drug-induced small intestinal injury in mice (2014)
  • Author(s): Higashimura, Y. Naito, Y. Takagi, T. Tanimura, Y. Mizushima, K. Harusato, A. Fukui, A. Yoriki, H. Handa, O. Ohnogi, H. Yoshikawa, T.
  • Journal Title: J Gastroenterol Hepatol Volume: 29 Issue: 2 Pages: 310-317
  • DOI: 10.1111/jgh.12373
  • NAID: 110009917750
  • Peer Reviewed
• [Journal Article] Potential role of oxidative protein modification in energy metabolism in exercise (2014)
  • Author(s): Aoi, W. Naito, Y. Yoshikawa, T.
  • Journal Title: Subcell Biochem Volume: 77 Pages: 175-180
  • DOI: 10.1007/978-94-007-7920-4_15
  • ISBN: 9789400779198, 9789400779204
  • Peer Reviewed
• [Presentation] 炎症病態とSPARCの関与についての検討 (2015)
  • Author(s): 田中 信、髙木智久、内山和彦、堀田祐馬、水島かつら、東村泰希、岡山哲也、鎌田和浩、堅田和弘、石川 剛、半田 修、内藤裕二、伊藤義人.
  • Organizer: 第57回日本消化器病学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-10-09
• [Presentation] 大腸炎症病態におけるSecreted Protein Acidic and Rich in Cystein (SPARC)の役割 (2015)
  • Author(s): 田中 信，髙木智久，内山和彦，豊川優希，堀田祐馬，水島かつら，東村泰希，岡山哲也，堅田和弘，半田 修，石川 剛，内藤裕二，伊藤義人．
  • Organizer: 第52回日本消化器免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-31
• [Presentation] マウス実験腸炎モデルにおけるSPARCの役割 (2015)
  • Author(s): 田中 信、髙木智久、内藤裕二、内山和彦、堀田祐馬、水島かつら、東村泰希、吉田直久、鎌田和浩、堅田和弘、石川 剛、半田 修、小西英幸、伊藤義人.
  • Organizer: 第101回日本消化器病学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2015-04-24
• [Presentation] Astaxanthin is a mitochondreal nutrient
  • Author(s): Naito Y
  • Organizer: 8th Asia Pacific Conference on Clinical Nutrition
  • Place of Presentation: Tokyo
• [Presentation] Biological role and measurement of advanced glycation ebd products and their precursors.
  • Author(s): Naito Y, Nakanishi M, Suehiro A, Mukai J, Uchida K.
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka
• [Presentation] 抗酸化療法の臨床応用への飛躍
  • Author(s): 内藤裕二，角田圭雄，吉川敏一
  • Organizer: 第13回日本抗加齢医学会総会
  • Place of Presentation: 横浜
• [Presentation] HO-1高発現M2マクロファージを標的にしたIBD治療
  • Author(s): 内藤裕二，髙木智久，内山和彦，堅田和弘，東村泰希
  • Organizer: 第50回日本消化器免疫学会総会
  • Place of Presentation: 東京
• [Book] 消化管（おなか）は泣いています (2016)
  • Author(s): 内藤裕二
  • Total Pages: 253
  • Publisher: ダイヤモンド社
• [Book] 人生を変える賢い腸のつくり方 (2016)
  • Author(s): 内藤裕二
  • Total Pages: 253
  • Publisher: ダイヤモンド社
• [Book] 機能性食品・素材と運動療法-生活習慣病予防と運動機能維持，向上を目指して- (2013)
  • Author(s): 内藤裕二，青井 渉，髙木智久，吉川敏一
  • Total Pages: 350
  • Publisher: シーエムシー出版
• [Remarks] 京都府立医科大学消化器内科消化管研究室
  • URL: http://www.f.kpu-m.ac.jp/k/syokanai/reseaches/shokakan.html
• [Patent(Industrial Property Rights)] 大腸がんの検出方法 (2015)
  • Inventor(s): 内藤裕二
  • Industrial Property Rights Holder: 内藤裕二
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-026486
  • Filing Date: 2015-02-13