A New Therapeutic Approach Using a Schizophyllan-based Drug Delivery System for Inflammatory Bowel Disease

• Project/Area Number: 25460963
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kurume University
• Principal Investigator: TAKEDATSU HIDETOSHI 久留米大学, 医学部, 助教 (80352144)
• Co-Investigator(Renkei-kenkyūsha): MOCHIZUKI Shinichi 北九州市立大学, 国際環境工学部, 講師 (10520702)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 炎症性腸疾患 / ドラッグデリバリーシステム / アンチセンス / シゾフィラン

Outline of Final Research Achievements

Antisense technologies for the inhibition of gene expression provide an effective strategy in inflammatory bowel disease (IBD). We have developed a delivery system for antisense using schizophyllan (SPG), a polysaccharide that belongs to the β- glucan family. This system has several advantages enabling the effective suppression of targeted RNA or DNA: SPG complex is stable and effectively taken up into macrophages through Dectin-1.
TNF-alpha mainly produced by macrophages has been shown to have a pathogenetic role in IBD. The effect of antisense TNF-alpha/SPG complex to intestinal macrophages of DSS colitis was shown the increase of uptake into macrophages and the suppression of TNF-alpha. The rectal administration of antisense TNF-alpha/SPG complex effectively suppressed TNF-alpha production and significantly ameliorated intestinal inflammation.
Our result demonstrated the usefulness of local administered antisense/SPG as new therapeutic approach in IBD.

Research Products

• [Journal Article] Nanomedicine and drug delivery strategies for treatment of inflammatory bowel disease (2015)
  • Author(s): Hidetoshi Takedatsu, Keiichi Mitsuyama, Takuji Torimura
  • Journal Title: World Journal of Gastroenterology Volume: 21 Issue: 40 Pages: 11343-11352
  • DOI: 10.3748/wjg.v21.i40.11343
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 炎症性腸疾患におけるDrug delivery systemを利用したアンチセンス治療効果の検討 (2016)
  • Author(s): 竹田津英稔、光山慶一、鳥村拓司
  • Organizer: 第102回日本消化器病学会総会 パネルディスカッション
  • Place of Presentation: 京王プラザ（東京都新宿区）
  • Year and Date: 2016-04-21
• [Presentation] 炎症性腸疾患マウスモデルにおけるDrug delivery systemを利用したアンチセンス治療効果の検討 (2015)
  • Author(s): 竹田津英稔、光山慶一、鳥村拓司
  • Organizer: 第52回日本消化器免疫学会総会 シンポジウム
  • Place of Presentation: 京王プラザ（東京都新宿区）
  • Year and Date: 2015-07-30