Metabolomic profile of hepatitis C virus-infected hepatocytes
Project/Area Number |
25460974
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | Yamagata University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SHO Li 山形大学, 医学(系)研究科, 助教 (80344787)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | HCV / メタボローム / 細胞内代謝 / 感染 / メタボローム解析 / 感染培養系 |
Outline of Final Research Achievements |
Hepatitis C virus (HCV) infection causes dramatic changes within infected hepatocytes. The aim of this study was to analyze the effect HCV infection has on the hepatocyte metabolome between HCVcc-infected and uninfected Huh7.5.1 cells in vitro using mass spectrometer for metabolomic analysis. More than 500 metabolites were identified and quantified by comparison of the uninfected hepatocytes. In total, 63 metabolites were siginificantly increased, 3 (citrate, cis-aconitate and iso-citrate) decreased relative to control. Based on the findings of this study, it would appear that many different metabolomics pathways are disrupted as a result of HCV infection, most notably glycolysis and TCA cycle metabolism. These results highlight a number of previously unreported metabolic interactions and give a more in depth into the effect that HCV has on host cell biochemical processes.
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] A thymine-adenine dinucleotide repeat polymorphism near IL28B is associated with spontaneous clearance of hepatitis C virus.2015
Author(s)
Hiramine S, Sugiyama M, Furusyo N, Uto H, Ido A, Tsubouchi H, Watanabe H, Ueno Y, Korenaga M, Murata K, Masaki N, Hayashi J, Thomas DL, Mizokami M
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Journal Title
J Gastroenterol
Volume: 51
Pages: 1069-1077
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dynamics of serum metabolites in patients with chronic hepatitis C receiving pegylated interferon plus ribavirin: A metabolomics analysis.2013
Author(s)
Saito T, Sugimoto M, Igarashi K, Saito K, Shao L, Katsumi T, Tomita K, Sato C, Okumoto K, Nishise Y, Watanabe H, Tomita M, Ueno Y, Soga T.
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Journal Title
Metabolism
Volume: 62
Pages: 1577-1586
Related Report
Peer Reviewed
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[Journal Article] Impaired mitochondrial ß-oxidation in patients with chronic hepatitis C: relation with viral load and insulin resistance.2013
Author(s)
Sato C, Saito T, Shao L, Okumoto K, Nishise Y, Watanabe H, Makino N, Fukao A, Kitanaka C, Kayama T, Ueno Y, Kawata S.
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Journal Title
BMC Gastroenterol
Volume: 13
Pages: 112-119
Related Report
Peer Reviewed
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[Journal Article] Serum prolactin levels and prolactin mRNA expression in peripheral blood mononuclear cells in hepatitis C vrus infection.2013
Author(s)
Ishii R, Saito T, Shao L, Okumoto K, Nishise Y, Watanabe H, Makino N, Fukao A, Kitanaka C, Kayama T, Ueno Y, Kawata S.
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Journal Title
J Med Virol
Volume: 85
Pages: 1199-1205
Related Report
Peer Reviewed
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[Journal Article] A case of adult type 1 Gaucher disease complicated by temporal intestinal hemorrhage.2013
Author(s)
Ito J, Saito T, Numakura C, Iwaba A, Sugahara S, Ishii R, Sato C, Haga H, Okumoto K, Nishise Y, Watanabe H, Ida H, Hayasaka K, Togashi H, Kawata S, Ueno Y.
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Journal Title
Case Rep Gastroenterol
Volume: 7
Pages: 340-346
Related Report
Peer Reviewed
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[Presentation] Association between (TA)n dinucleotide repeat near IL28B gene and HCV spontaneous clearance in Japanese and African American.2014
Author(s)
Sugiyama M, Hiramine S, Furusyo N, Ido A, Tsubouchi H, Watanabe H, Ueno Y, Korenaga M, Murata K, Masaki N, Kanto T, Hayashi J, Thomas D, Mizokami M.
Organizer
The 65th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Hynes Convention Center, Boston, USA
Year and Date
2014-11-07 – 2014-11-11
Related Report
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[Presentation] Ability of miRNA profiling to distinguish the clinical course of patients with primary biliary cirrhosis: comprehemsive analysis with deep sequencing.2013
Author(s)
Katsumi T, Ninomiya M, Mizuno K, Tomita K, Saito C, Okumoto K, Nishise Y, Watanabe H, Saito T, Shimosegawa T, and Ueno Y.
Organizer
The 64th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Washington Convention Center, Washington DC(USA)
Related Report
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[Presentation] Polymorphisms consisting of (TA)n dinucleotide repeat near IL28B gene could improve the predictive value for HCV spontaneous clearance with IL28B SNPs.2013
Author(s)
Sugiyama M, Hiramine S, Furusho N, Ido A, Tsubouchi H, Watanabe H, Ueno Y, Korenaga M, Murata K, Masaki N, and Mizokami M.
Organizer
The 64th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Washington Convention Center, Washington DC(USA)
Related Report
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[Presentation] Epiregulin promotes the emergence and proliferation of adult liver progenitor cells.2013
Author(s)
Tomita K, Haga H, Mizuno K, Katsumi T, Tomita K, Saito C, Okumoto K, Nishise Y, Watanabe H, Saito T, and Ueno Y.
Organizer
The 64th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Washington Convention Center, Washington DC(USA)
Related Report
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[Book] メディカルレビュー2013
Author(s)
渡辺久剛、斎藤貴史、石橋正道、新澤陽英、河田純男
Total Pages
175
Publisher
コホート研究からみたウイルス性肝炎の解明
Related Report
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[Book] ハリソン内科学第4版2013
Author(s)
渡辺久剛、冨田恭子、河田純男
Total Pages
3120
Publisher
メディカル・サイエンス・インターナショナル
Related Report
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[Book] 肝胆膵2013
Author(s)
渡辺久剛、上野義之
Total Pages
564
Publisher
アークメディカル
Related Report