The role of NK cell in the patients with hepatitis C virus infection; analysis focusing on NK cell receptor and IFN signal transducer
| Project/Area Number |
25460993
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Tatsumi Tomohide 大阪大学, 大学院医学系研究科, 講師 (20397699)
Miyagi Takuya 大阪大学, 大学院医学系研究科, 助教 (80532986)
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| Research Collaborator |
Yoshioka Teppei 大阪大学, 大学院医学系研究科, 大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | C型慢性肝炎 / NK細胞 / C型肝炎 |
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| Outline of Final Research Achievements |
The activation of NK cells was controlled by various NK cell receptors, however, the relationship between NK cell receptors and HCV infection remains still unclear.
Peripheral blood lymphocytes were obtained from patients with HCV infection (CHC; n=50) and healthy subjects (HS; n=35). The expression of NKp46 (an activating receptor)/NKG2A (an inhibitory receptor) were analyzed by flow cytometry. A unique NK cell subset strongly expressed both NKp46 and NKG2A was identified (NKp46highNKG2Ahigh subset). The frequencies of this subset were higher in CHC (10.3%) than in HS (5.9%), and positively correlated with serum ALT level (P <0.01, r = 0.432). The expression of Fas-L, STAT1, TRAIL and CD107a were higher in this subset than in the other. The expression of IFN-γ were lower in this subset than in the other. Phosphorylation of STAT1 was higher in this subset than in the other.NKp46highNKG2Ahigh NK cell subset exhibited high cytotoxicity and associated with liver injury in HCV infection.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] NK cells play important roles in the pathogenesis of HBV infection via alternation of NKp46high NKG2Ahigh subset2015
Author(s)
Teppei Yoshioka, Yoshinobu Yokoyama, Akira Nishio, Yoshiki Onishi, Kaori Mukai, Satoshi Aono, Minoru Shigekawa, Hayato Hikita, Ryotaro Sakamori, Naoki Hiramatsu, Takuya Miyagi, Tomohide Tatsumi and Tetsuo Takehara
Organizer
The 65th Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
San Francisco（米国）
Year and Date
2015-11-16
Related Report
Int'l Joint Research
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[Presentation] Evaluation of both Fucosylated haptoglobin and Mac-2 binding protein as new biomarkers to estimate liver fibrosis in chronic hepatitis C patients2015
Author(s)
Seiichi Tawara, Tomohide Tatsumi, Sadaharu Iio, Ichizo Kobayashi, Shigeki Suemura, Atsuo Takigawa, Tadashi Kegasawa, Takahiro Suda, Teppei Yoshioka, Yoshiki Onishi, Akira Nishio, Satoshi Aono, Minoru Shigekawa, Hayato Hikita, Ryotaro Sakamori, Naoki Hiramatsu, Eiji Miyoshi, Tetsuo Takehara.
Organizer
The 65th Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
San Francisco（米国）
Year and Date
2015-11-16
Related Report
Int'l Joint Research
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