Development of method to detect four mRNAs of hepatitis B virus in liver tissue
| Project/Area Number |
25461004
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Nagoya City University |
|---|
Principal Investigator |
FUJIWARA Kei 名古屋市立大学, 大学院医学研究科, 講師 (70635804)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
新海 登 名古屋市立大学, 大学院医学研究科, 研究員 (30543988)
|
|---|
| Research Collaborator |
KATO Takanobu
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | HBV / RNA / B型肝炎 / B型肝炎ウイルス |
|---|
| Outline of Final Research Achievements |
In this study, a novel method to quantify four different mRNA of hepatitis B virus (HBV) was searched. To perform real-time PCR, sets of primers and probes were designed, and the conditions for real-time PCR was determined, and then the most stable and better methods for quantification of HBV mRNAs were searched and determined. Then, methods to purify and concentrate nucleic acid which were substrate for real-time PCR reaction were developed. Futhermore, pre-amplification PCR to improve the sensitivity was developed. The relationship of HBV mRNAs and serological marker of clinical disease will be analyzed.
|
Report
(5 results)
Research Products
(21 results)
-
[Journal Article] Clinical evaluation of sofosbuvir/ledipasvir in chronic hepatitis C genotype 1 with and without prior daclatasvir/asnaprevir therapy.2017
Author(s)
Iio E, Shimada N, Takaguchi K, Senoh T, Eguchi Y, Atsukawa M, Tsubota A, Abe H, Kato K, Kusakabe A, Miyaki T, Matsuura K, Matsunami K, Shinkai N, Fujiwara K, Nojiri S, Tanaka Y.
-
Journal Title
Hepatol Res.
Volume: 印刷中
Issue: 12
Pages: 1308-1316
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] A novel glycobiomarker, Wisteria floribunda agglutinin macrophage colony-stimulating factor receptor, for predicting carcinogenesis of liver cirrhosis.2016
Author(s)
Iio E, Ocho M, Togayachi A, Nojima M, Kuno A, Ikehara Y, Hasegawa I, Yatsuhashi H, Yamasaki K, Shimada N, Ide T, Shinkai N, Nojiri S, Fujiwara K, Joh T, Mizokami M, Narimatsu H, Tanaka Y.
-
Journal Title
Int J Cancer
Volume: 138(6)
Issue: 6
Pages: 1462-1471
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Influence of genes suppressing interferon effects in peripheral blood mononuclear cells during triple antiviral therapy for chronic hepatitis C.2015
Author(s)
Iijima S, Matsuura K, Watanabe T, Onomoto K, Fujita T, Ito K, Iio E, Miyaki T, Fujiwara K, Shinkai N, Kusakabe A, Endo M, Nojiri S, Joh T, Tanaka Y.
-
Journal Title
PLoS One.
Volume: 10
Issue: 2
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] ITPA genetic variants influence efficacy of PEG-IFN/RBV therapy in older patients infected with HCV genotype 1 and favourable IL28B type.2014
Author(s)
Matsuura K, Tanaka Y, Watanabe T, Fujiwara K, Orito E, Kurosaki M, Izumi N, Sakamoto N, Enomoto N, Yatsuhashi H, Kusakabe A, Shinkai N, Nojiri S, Joh T, Mizokami M.
-
Journal Title
J Viral Hepat.
Volume: 21
Issue: 7
Pages: 466-474
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
