Experimental and clinical studies on the effects of L-carnitine in minimal hepatic encephalopathy
| Project/Area Number |
25461009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Sawara Kei 岩手医科大学, 内科学講座消化器内科肝臓分野, 助教 (40405816)
Wang Ting 岩手医科大学, 内科学講座消化器内科肝臓分野, 助教 (70416171)
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| Research Collaborator |
Kakisaka Keisuke 岩手医科大学, 内科学講座消化器内科肝臓分野, 助教
Kuroda Hidekatsu 岩手医科大学, 内科学講座消化器内科肝臓分野, 助教
Kato Akinobu 盛岡市立病院, 院長
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | カルニチン / ミニマル肝性脳症 / 肝硬変 / アンモニア / 血清遊離脂肪酸 / アストロサイト / 肝性脳症 |
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| Outline of Final Research Achievements |
Clinical study: The levels of serum carnitine in patients with liver cirrhosis (LC) were almost within normal range. Approximately 11% of LC patients had serum CA levels < 45μmol/L. The serum CA levels did not correlate with severity of liver damage and the existence of MHE, but were closely associated with renal dysfunction. The serum free fatty acid levels strongly correlated with serum Ac-CA levels and the Ac-CA to T-CA ratio. The improvement of hyperammonemia was observed 46% of LC patients administrated L-CA.
Experimental study: Cell viability and intracellular total ROS and LDH leakage of human astrocytes were significantly increased after treatment with NH4Cl, while cotreatment with L-CA significantly improved these cytotoxic changes. In metabolome analysis, although the expression levels of the all essential amino acids showed high in the NH4Cl-treated cells, these changes were improved in cotreatment with L-CA. L-CA may protect astrocytes against the toxic effects of NH4Cl.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Branched-chain amino acids prevent hepatocarcinogenesis and prolong survival of patients with cirrhosis2015
Author(s)
Kawaguchi T, Shiraishi K, Ito T, Suzui K, Koreeda C, Ohtake T, Iwasa M, Tokumoto Y, Endo R, Kawamura N, Shiraki M, Habu D, Tsuruta S, Miwa Y, Kawaguchi A, Kakuma T, Sakai H, Kawada N, Hanai T, Takahashi S, Kato A, Onji M, Takei Y, Kohgo Y, Seki T, Tamano M, Katayama K, Mine T, Sata M, Moriwaki H, and Suzuki K
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Journal Title
Clin Gastroenterol Hepatol
Volume: 12
Issue: 6
Pages: 1012-1018
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of zinc on liver cirrhosis with hyperammonemia: A preliminary randomized, placebo-controlled, double-blind trial2014
Author(s)
Katayama K, Saito M, Kawaguchi T, Endo R, Sawara K, Nishiguchi S, Kato A, Kohgo H, Suzuki K, Sakaida I, Ueno Y, Habu D, Ito T, Moriwaki H, Suzuki K
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Journal Title
Nutrition
Volume: 30
Pages: 1409-1419
Related Report
Peer Reviewed / Open Access
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[Journal Article] 肝疾患 肝性脳症2014
Author(s)
鈴木一幸、佐原 圭、遠藤龍人、滝川康裕
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Journal Title
日本臨牀 内科疾患にみられる神経障害（疾患）肝・腎・肺疾患
Volume: 別冊
Pages: 350-353
Related Report
Open Access
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