| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We previously reported that BART inhibited pancreatic cancer cell invasion and metastasis. This project seeks to identify the mechanisms by which BART inhibits the invasiveness and metastasis through regulating specific small G-proteins. The data provided here could provide important information to aid to improve the clinical outcome of pancreatic cancer patients. We determined the binding sites of two small G-proteins ARL2 and Rac1 with endogenous BART in pancreatic cancer cells. Furthermore, a specific ARL2-peptide and a specific Rac-1 peptide that could inhibit their binding with BART were found. Each peptide functioned in altering the activities of ARL2 and Rac1, and thereby inhibited pancreatic cancer cell motility and invasion. These discovery based studies will provide insight into the manner in which BART acts on the specific small G-proteins that are important to the motility and invasiveness of pancreatic cancer cells.
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