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Regulation of nestin using microRNA: Examination for clinical application of pancreatic cancer therapy

Research Project

Project/Area Number 25461027
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionTokyo Metropolitan Geriatric Hospital and Institute of Gerontology (2015)
Nippon Medical School (2013-2014)

Principal Investigator

Ishiwata Toshiyuki  地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究部長 (90203041)

Co-Investigator(Kenkyū-buntansha) MATSUDA YOKO  地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (20363187)
MATSUSHITA AKIRA  日本医科大学, 医学部, 助教 (70449263)
SHINJI SEIICHI  日本医科大学, 医学部, 助教 (80409193)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords膵癌 / 癌幹細胞 / miRNA / スフェア形成能 / nestin / 分子標的治療 / Nestin / microRNA
Outline of Final Research Achievements

Pancreatic cancer is associated with extremely high mortality rates, therefore, there is an urgent need to develop new therapeutic options. Nestin is a member of class VI intermediate filament proteins, and is known as a pancreatic cancer stem cell marker. Nestin plays important roles in proliferation, invasion and metastasis of pancreatic cancer cells. In the present study, we tried to identify the microRNAs (miRNAs) which are correlated with stemness of pancreatic cancer. Among 1190 miRNAs, we identified 8 types of miRNA that decrease the number of spheres, and 1 miRNA (miR-4254) that increases the number of spheres. Transfection of miR-4254-expression vector to PANC-1 cells increased nestin and nanog mRNA levels in the cells. These findings suggest that miR-4254 takes part in regulation of the stemness of pancreatic cancer.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Administration of small interfering RNA targeting human nestin inhibits pancreatic cancer cell proliferation and metastasis.2016

    • Author(s)
      Matsuda Y, Ishiwata T, Yoshimura H, Yamashita S, Ushijima T, Arai T.
    • Journal Title

      Pancreas

      Volume: 45 Issue: 1 Pages: 93-100

    • DOI

      10.1097/mpa.0000000000000427

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of fibroblast growth factor receptor 2 attenuates proliferation and invasion of pancreatic cancer.2014

    • Author(s)
      Matsuda Y, Yoshimura H, Suzuki T, Uchida E, Naito Z, Ishiwata T.
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 9 Pages: 1212-1219

    • DOI

      10.1111/cas.12470

    • Description
      in press
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rγ(null) (NOG) mice2014

    • Author(s)
      Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M and Ishiwata T
    • Journal Title

      American Journal of Pathology

      Volume: 184 Pages: 674-85

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of Thr(1495) of nestin in a mouse model of cerebral ischemia and reperfusion damage2013

    • Author(s)
      Matsuda Y, Suzuki G, Kusano T, Kawamoto Y, Yoshimura H, Fuse A, Yokota H, Naito Zand Ishiwata T
    • Journal Title

      Pathology International

      Volume: 63 Issue: 9 Pages: 448-56

    • DOI

      10.1111/pin.12092

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 癌幹細胞と上皮間葉転換を標的とした革新的な癌治療法2015

    • Author(s)
      石渡 俊行
    • Organizer
      第61回日本病理学会秋期特別総会
    • Place of Presentation
      東京大学安田講堂(東京都文京区)
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京都健康長寿医療センター研究所 研究チーム紹介 高齢者がん研究

    • URL

      http://www.tmghig.jp/J_TMIG/kenkyu/team/koureishagan.html

    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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