Differentiation of human iPS cells into insulin producing-cells using Sendai virus vectors

• Project/Area Number: 25461030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: ID Pharma
• Principal Investigator: SAEKI KOICHI 株式会社IDファーマ, DNAVECセンター・基礎研究部, 研究員 (40360052)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 糖尿病 / 細胞移植 / iPS細胞 / センダイウイルスベクター / 血糖値

Outline of Final Research Achievements

Sendai virus (SeV) vectors can efficiently introduce foreign genes into various organs without genomic insertion of transgenes and are expected to be clinically applicable. We improved SeV vectors for transient expression so that the vectors can be easily removed from the cells at nonpermissive temperatures. We obtained insulin producing-cells from induced pluripotent stem cells (iPSCs) using SeV vectors. These vectors enabled an efficient production of differentiated cells from iPSCs.

Research Products

• [Journal Article] Pro- vs anti-stenotic capacities of type-I vs type-II human induced pluripotent-derived endothelial cells (2015)
  • Author(s): Miwako Nishio, Masako Nakahara, Koichi Saeki, Katsuhito Fujiu, Hiroshi Iwata, Ichiro Manabe, Akira Yuo, Kumiko Saeki
  • Journal Title: World J Transl Med Volume: 4(3) Issue: 3 Pages: 113-122
  • DOI: 10.5528/wjtm.v4.i3.113
  • Peer Reviewed / Open Access
• [Journal Article] p38 mitogen-activated protein kinase regulates type-I vs type-II phenotyping of human vascular endothelial cells (2015)
  • Author(s): Masako Nakahara, Miwako Nishio, Koichi Saeki, Akira Yuo, Kumiko Saeki
  • Journal Title: World J Transl Med Volume: 4(3) Issue: 3 Pages: 101-112
  • DOI: 10.5528/wjtm.v4.i3.101
  • Peer Reviewed / Open Access
• [Journal Article] New categorization of human vascular endothelial cells by pro- vs anti-proliferative phenotypes (2015)
  • Author(s): Miwako Nishio, Masako Nakahara, Chikako Sato, Koichi Saeki, Hidenori Akutsu, Akihiro Umezawa, Kazuyuki Tobe, Kazuki Yasuda, Akira Yuo, Kumiko Saeki
  • Journal Title: World J Transl Med Volume: 4(3) Issue: 3 Pages: 88-100
  • DOI: 10.5528/wjtm.v4.i3.88
  • Peer Reviewed / Open Access
• [Journal Article] センダイウイルスベクターを用いた体細胞リプログラミング法の改良と臨床用キットの開発 (2015)
  • Author(s): 川口実太郎、佐伯晃一
  • Journal Title: BIO Clinica Volume: 30 Pages: 453-454
• [Journal Article] より進歩した体細胞リプログラミング法：センダイウイルスベクター(CytoTune-iPS)による進歩 (2014)
  • Author(s): 川口実太郎、伴浩志、佐伯晃一
  • Journal Title: 細胞 Volume: 46 Pages: 229-230
• [Presentation] 一過性発現SeVベクターを用いたiPS細胞の誘導 (2016)
  • Author(s): 佐伯晃一、川口実太郎、草野好司、井上誠
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17
• [Presentation] 臨床用iPS細胞作製センダイベクターセットGMP-CytoTune-iPSの開発 (2016)
  • Author(s): 川口実太郎、弘中孝史、上田泰治、佐伯晃一、草野好司、井上誠、朱亜峰
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17
• [Presentation] SeVベクターの改良：in vitroでの一過性発現 (2015)
  • Author(s): 佐伯晃一、川口実太郎、草野好司、井上誠
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01