Differentiation of human iPS cells into insulin producing-cells using Sendai virus vectors
| Project/Area Number |
25461030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | ID Pharma |
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Principal Investigator |
SAEKI KOICHI 株式会社IDファーマ, DNAVECセンター・基礎研究部, 研究員 (40360052)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 糖尿病 / 細胞移植 / iPS細胞 / センダイウイルスベクター / 血糖値 |
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| Outline of Final Research Achievements |
Sendai virus (SeV) vectors can efficiently introduce foreign genes into various organs without genomic insertion of transgenes and are expected to be clinically applicable. We improved SeV vectors for transient expression so that the vectors can be easily removed from the cells at nonpermissive temperatures. We obtained insulin producing-cells from induced pluripotent stem cells (iPSCs) using SeV vectors. These vectors enabled an efficient production of differentiated cells from iPSCs.
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Report
(4 results)
Research Products
(8 results)
