The Mechanisms of Adipose Tissue Derived Regenerative Cells for Ischemic Heart Failure

• Project/Area Number: 25461049
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Kanazawa University
• Principal Investigator: Takamura Masayuki 金沢大学, 大学病院, 講師 (60362000)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医療 / 脂肪組織由来再生細胞 / 虚血性心疾患 / サイトカイン / 遺伝子発言解析 / 脂肪由来再生細胞 / 遺伝子発現解析 / 重症心不全

Outline of Final Research Achievements

The analysis of adipose tissue derived regenerative cell were performed in patients who underwent our clinical study entitled “The Effect of Autologous Adipose Tissue Derived Regenerative Cells to Ischemic Heart Failure Patient”. Part of the beneficial effects to improve the cardiac performance may be related with reduction of inflammatory cytokines such as IL-1B, TNF-A.Also we investigated the effect of adipose tissue derived regenerative cells to myocardial ischemia-reperfusion model mice. And the significant reduction of inflammatory cytokines and improved cardiac function were closely related with the infusion of regenerative cells.
Part of these findings were presented at The 80th Annual Scientific Meeting of Japanese Circulation Society and The 15th Congress of The Japanese Society for Regenerative Medicine.

Research Products

• [Journal Article] Single-Unit Muscle Sympathetic Nerve Activity Reflects Sleep Apnea Severity, Especially in Severe Obstructive Sleep Apnea Patients. (2016)
  • Author(s): Hamaoka T, Murai H, Kaneko S, Usui S, Okabe Y, Tokuhisa H, Kato T, Furusho H, Sugiyama Y, Nakatsumi Y, Takata S, Takamura M.
  • Journal Title: Front Physiol. Volume: 7 Pages: 66-66
  • DOI: 10.3389/fphys.2016.00066
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Usefulness of Electrocardiographic Voltage to Determine Myocardial Fibrosis in Hypertrophic Cardiomyopathy. (2016)
  • Author(s): Konno T, Nagata Y, Teramoto R, Fujino N, Nomura A, Tada H, Sakata K, Furusho H, Takamura M, Nakamura H, Kawashiri MA, Yamagishi M, Hayashi K.
  • Journal Title: Am J Cardiol. Volume: 117 Pages: 443-449
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Inflammation of left atrial epicardial adipose tissue is associated with paroxysmal atrial fibrillation. (2015)
  • Author(s): Kusayama T, Furusho H, Kashiwagi H, Kato T, Murai H, Usui S, Kaneko S, Takamura M.
  • Journal Title: J Cardiol. Volume: 15 Issue: 5 Pages: 371-378
  • DOI: 10.1016/j.jjcc.2015.11.005
  • Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Landiolol, an ultra-short-acting β1-blocker, is useful for managing supraventricular tachyarrhythmias in sepsis (2015)
  • Author(s): Masaki Okajima, Masayuki Takamura, Takumi Taniguchi
  • Journal Title: World J Crit Care Med Volume: 4 Issue: 3 Pages: 251-257
  • DOI: 10.5492/wjccm.v4.i3.251
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 1.Differential effects of azelnidipine and amlodipine on sympathetic nerve activity in patients with primary hypertension. (2014)
  • Author(s): 1.JI Inomata, H Murai, S Kaneko, T Hamaoka, T Ikeda, D Kobayashi, S Usui, H Furusho, Y Sugiyama, S Takata, M Takamura
  • Journal Title: J Hypertens. Volume: 32(9) Issue: 9 Pages: 1898-904
  • DOI: 10.1097/hjh.0000000000000270
  • Peer Reviewed
• [Journal Article] Separated right and left ventricular excitation during right ventricular septal pacing in a patient with narrow QRS wave: a case report. (2014)
  • Author(s): 2.T Yaegashi, H Furusho, A Chikata, S Usui, S Kaneko, M Yamagishi, M Takamura
  • Journal Title: J Med Case Rep Volume: 8(1) Issue: 1 Pages: 158-158
  • DOI: 10.1186/1752-1947-8-158
  • NAID: 120005463336
  • Peer Reviewed
• [Journal Article] MuRF1 negatively regulates pathological cardiac hypertrophy through downregulation of calcineurin A (2014)
  • Author(s): M.Yokota（他8名、7番目） et al
  • Journal Title: Circ Heart Fail Volume: 7 Issue: 3 Pages: 479-490
  • DOI: 10.1161/circheartfailure.113.000713
  • Peer Reviewed / Open Access
• [Journal Article] Rho-kinase activation in leukocytes plays a pivotal role in myocardial ischemia/reperfusion injury (2014)
  • Author(s): Kitano K, Usui S, Ootsuji H, Takashima S, Kobayashi D, Murai H, Furusho H, Nomura A, Kaneko S, Takamura M.
  • Journal Title: PLoS One. Volume: 9 Issue: 3 Pages: e92242-e92242
  • DOI: 10.1371/journal.pone.0092242
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Coronary vessel floating sign and vasospastic angina in a patient with cardiac lymphoma. (2014)
  • Author(s): 5.Chikata A, Sakagami S, Kanamori N, Kato C, Omi W, Saeki T, Nagai H, Kawashima A, Usui S, Takamura M
  • Journal Title: Int J Cardiol Volume: 178(1) Issue: 1 Pages: e20-e25
  • DOI: 10.1016/j.ijcard.2014.06.081
  • Peer Reviewed
• [Journal Article] Adipose tissue derived stromal stem cell therapy in murine ConA-derived hepatitis is dependent on myeloid-lineage and CD4+ T-cell suppression. (2013)
  • Author(s): Higashimoto M, Sakai Y, Takamura M, Usui S, Nasti A, Yoshida K, Seki A, Komura T, Honda M, Wada T, Furuichi K, Ochiya T, Kaneko S.
  • Journal Title: Eur J Immunol Volume: 11 Pages: 2956-68
  • Peer Reviewed
• [Presentation] Insulin Resistance Predicts the Yield of Therapeutic Stem Cells in Adipose Tissue for Autologous GraXs in Patients with Cardiovascular Disease. (2016)
  • Author(s): Oto Inoue, Soichiro Usui, Mitsuhiro Sugita, Shin-‐ichiro Takashima, Takeshi Kato, Hisayoshi Murai Hiroshi Furusho, Hirofumi Takemura, Yoshio Sakai, Shuichi Kaneko, Masayuki Takamura
  • Organizer: 第80回日本循環器学会総会（仙台）
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-03-18
• [Presentation] Adipose Tissue-derived Stromal Cells Exert Beneficial Effect to Systemic Responses Following Myocardial Ischemia Reperfusion. (2016)
  • Author(s): Masayuki Takamura, Soichiro Usui, Oto Inoue, Hiroshi Ootsuji, Shin-ichiro Takashima, Shintaro Matsuura, Mitsuhiro Sugita, Ayano Nomura, Takeshi Kato, Hisayoshi Murai, Hiroshi Furusho, Yoshio Sakai, Shuichi Kaneko
  • Organizer: 第80回日本循環器学会総会（仙台）
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-03-18
• [Presentation] 脂肪組織慢性炎症が間葉系幹細胞プロファイルに及ぼす影響 (2016)
  • Author(s): 井上 己音 薄井 荘一郎 杉田 光洋 高島 伸一郎 加藤 武史 村井 久純 古荘 浩司 酒井 佳夫 竹村 博文 金子 周一 高村 雅之
  • Organizer: 第15回日本再生医療学会総会（大阪）
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17
• [Presentation] 脂肪由来間葉系細胞投与による心筋虚血再灌流後の全身応答への影響 (2016)
  • Author(s): 薄井 荘一郎 高村 雅之 井上 己音 高島 伸一郎 酒井 佳夫 金子 周一
  • Organizer: 第15回日本再生医療学会総会（大阪）
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17
• [Remarks] 恒常性制御学（旧第一内科） 金沢大学付属病院内科
  • URL: http://www.m-kanazawa.jp/