A comprehensive understanding of the role of a non-neuronal cholinergic system in the pathology of myocardial infarction
Project/Area Number |
25461059
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cardiovascular medicine
|
Research Institution | Kochi University |
Principal Investigator |
ARIKAWA MIKIHIKO 高知大学, 教育研究部自然科学系, 准教授 (20432817)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 生体機能利用 / 心機能保護 / アセチルコリン / 非神経性コリン作動系 / 心筋梗塞 |
Outline of Final Research Achievements |
Cardiac fibroblasts (cFb) play an important role in structural and functional remodeling of the heart after myocardial infarction (MI). In the present study, we investigated the hypothesis that cFb possess the cholinergic system by which the cFb contribute to the cardioprotection through the release of acetylcholine (ACh). In vitro study showed that the cultured cFb isolated from adult mouse heart express ACh synthesis and degradation enzymes, ACh transporters, and various types of ACh receptors. In vivo immunohistochemical study showed that ChAT signals were co-localized with cFb markers, indicating that cFb express ChAT in the heart. Moreover, westernblot analysis showed that the expression of cFb ChAT was increased in only infarcted myocardium after the MI, resulted in the increase of cardiac ACh content. In conclusion, cFb produce ACh in infarcted myocardium after the MI, which may contribute to the cardioprotection during the post infarct cardiac remodeling process.
|
Report
(5 results)
Research Products
(13 results)
-
[Journal Article] Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1.2017
Author(s)
Funadani R*, Sogame Y*,(*equal contributor) Kojima K, Takeshita T, Yamamoto K, Tsujizono T, Suizu F, Miyata S, Yagyu KI, Suzuki T, Arikawa M, Matsuoka T
-
Journal Title
FEMS microbiology letters
Volume: 363
Issue: 18
Pages: fnw203-fnw203
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Donepezil Can Improve Ischemic Muscle Atrophy by Activating Angiomyogenic Properties of Satellite Cells2014
Author(s)
Noguchi T, Kakinuma Y, Arikawa M, Okazaki K, Hoshino E, Iiyama T, Kubo T, Kitaoka H, Doi Y, Sato T
-
Journal Title
Circulation Journal
Volume: 78
Issue: 9
Pages: 2317-2324
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
[Presentation] Elucidation of the role of the double-stranded RNA binding protein NF90-NF45 complex in muscular regeneration.2016
Author(s)
Hiroshi Todaka, Takuma Higuchi, Keiko Morisawa, Takeshi Miwa, Lai Sylvia Chin See, Masayuki Tsuda, Yasunori Sugiyama, Mikihiko Arikawa, Takayuki Sato, Shuji Sakamoto.
Organizer
RNA 2016
Place of Presentation
International Conference Center (Kyoto, Japan)
Year and Date
2016-06-28
Related Report
Int'l Joint Research
-
[Presentation] 心臓の底力2015
Author(s)
有川幹彦
Organizer
第108回土佐生物学会大会
Place of Presentation
高知大学(高知)
Year and Date
2015-12-12
Related Report
-
-
-