| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Cardiac fibroblasts (cFb) play an important role in structural and functional remodeling of the heart after myocardial infarction (MI). In the present study, we investigated the hypothesis that cFb possess the cholinergic system by which the cFb contribute to the cardioprotection through the release of acetylcholine (ACh). In vitro study showed that the cultured cFb isolated from adult mouse heart express ACh synthesis and degradation enzymes, ACh transporters, and various types of ACh receptors. In vivo immunohistochemical study showed that ChAT signals were co-localized with cFb markers, indicating that cFb express ChAT in the heart. Moreover, westernblot analysis showed that the expression of cFb ChAT was increased in only infarcted myocardium after the MI, resulted in the increase of cardiac ACh content. In conclusion, cFb produce ACh in infarcted myocardium after the MI, which may contribute to the cardioprotection during the post infarct cardiac remodeling process.
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