Plasma microRNAs as a new generation of biomarker for developing heart failure

• Project/Area Number: 25461075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Fujita Health University
• Principal Investigator: Hideo Izawa 藤田保健衛生大学, 医学部, 教授 (80402569)
• Co-Investigator(Kenkyū-buntansha): OZAKI Yukio 藤田保健衛生大学, 医学部, 教授 (50298569) ; YOKOI Hiroatsu 藤田保健衛生大学, 医学部, 講師 (20644876) ; FUJIWARA Wakaya 藤田保健衛生大学, 医学部, 講師 (60644870)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 循環器 / 心不全 / マイクロRNA / バイオマーカー

Outline of Final Research Achievements

Heart failure remains a major and growing public health problem in aging society like Japan. More than one million Japanese have suffered from heart failure, and almost 30% of the medical costs have been used in the treatment of cardiovascular disease. Cardiomyopathy including dilated cardiomyopathy (DCM) are among the most common underlying heart failure in Japan. It is therefore important that patients with cardiomyopathy who are refractory to standard medical treatment be placed under strict management as early as possible. Micro RNAs (miRNAs) are single stranded and short noncoding RNAs, generated from endogenous transcripts, and miRNAs are suggested to have important roles in a variety of physiological and pathological processes. Recently, circulating miRNAs has shown great promise as novel biomarkers for cardiovascular disease. In this study, we showed that the expression level of a miRNA was significantly increased in patients with DCM.

Research Products

• [Journal Article] Clinical Characteristics and Long-Term Outcomes of Hypertrophic Cardiomyopathy (2015)
  • Author(s): Yoshinaga M, Yoshikawa D, Ishii H, Hirashiki A, Okumura T, Kubota A, Sakai S, Harada K, Somura F, Mizuno T, Fujiwara W, Yokoi H, Hayashi M, Ishii J, Ozaki Y, Murohara T, Yoshida Y, Amano T, Izawa H.
  • Journal Title: International Heart Journal Volume: 56 Issue: 4 Pages: 415-420
  • DOI: 10.1536/ihj.14-418
  • NAID: 130005087575
  • ISSN: 1349-2365, 1349-3299
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Takotsubo Cardiomyopathy with Involvement of Delayed-onset Rhabdomyolysis and Acute Kidney Injury after Rosuvastatin Treatment (2015)
  • Author(s): Kamada T, Hayashi M, Yokoi H, Fujiwara W, Yoshikawa D, Mukaide D, Sugishita Y, Yoshinaga M, Ito T, Ozaki Y, Izawa H.
  • Journal Title: Internal Medicine Volume: 54 Issue: 1 Pages: 31-35
  • DOI: 10.2169/internalmedicine.54.3239
  • NAID: 130004902992
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Prescription patterns of oral anticoagulants for patients with non-valvular atrial fibrillation: experience at a Japanese single institution. (2015)
  • Author(s): Tagaya M, Yoshikawa D, Sugishita Y, Yamauchi F, Ito T, Kamada T, Yoshinaga M, Mukaide D, Fujiwara W, Yokoi H, Hayashi M, Watanabe E, Ishii J, Ozaki Y, Izawa H.
  • Journal Title: Heart and Vessels Volume: Epub ahead of print Pages: 0-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plasma MicroRNA-100 as a Biomarker of Coronary Plaque Vulnerability (2015)
  • Author(s): Izawa H, Amano T
  • Journal Title: Circulation Journal Volume: 79 Issue: 2 Pages: 303-304
  • DOI: 10.1253/circj.CJ-14-1295
  • NAID: 130004927076
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Multimarker Approach to Risk Stratification for Long-Term Mortality in Patients on Chronic Hemodialysis (2015)
  • Author(s): Junichi Ishii, Hiroshi Takahashi, Fumihiko Kitagawa, Atsuhiro Kuno, Ryuunosuke Okuyama, Hideki Kawai, Takashi Muramatsu, Hiroyuki Naruse, Sadako Motoyama, Shigeru Matsui, Midori Hasegawa, Toru Aoyama, Daisuke Kamoi, Hirotake Kasuga, Hideo Izawa, Yukio Ozaki, Yukio Yuzawa
  • Journal Title: Circulation Journal Volume: 79 Issue: 3 Pages: 656-663
  • DOI: 10.1253/circj.CJ-14-0915
  • NAID: 130004927120
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Plasma indoxyl sulfate and estimated glomerular filtration rate (2014)
  • Author(s): Yoshikawa D, Ishii H, Suzuki S, Takeshita K, Kumagai S, Hayashi M, Niwa T, Izawa H, Murohara T
  • Journal Title: Circulation Journal Volume: 78 Pages: 2477-2482
  • Peer Reviewed / Open Access
• [Journal Article] Role of the left atrial function on the pseudonormalization of the transmitral flow velocity pattern evaluated by two-dimensional tissue tracking technique (2013)
  • Author(s): Mukaide D, Tabata T, Kinoshita K, Yokoi H, Fujiwara W, Inami O, Sugishita Y, Ukai G, Yoshinaga M, Kamada T, Nomura M, Izawa H
  • Journal Title: J Cardiol Volume: 61 Issue: 5 Pages: 365-371
  • DOI: 10.1016/j.jjcc.2013.01.007
  • Peer Reviewed
• [Presentation] Combined Assessment of Acute Kidney Injury and Increased Plasma Concentration of D-dimer Is Useful for Evaluating 1-year Mortality in Patients Hospitalized to Coronary Care Units. (2015)
  • Author(s): Matsui S, Ishii J, Okuyama R, Takahashi H, Kawai H, Muramatsu T, Yamada A, Motoyama S, Naruse H, Hayashi M, Izawa H, Ozaki Y.
  • Organizer: Scientific Sessions 2015, American Heart Association.
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2015-11-07
  • Int'l Joint Research
• [Presentation] Combined Assessment of High-Sensitive Troponin I and N-Terminal Pro-B-Type Natriuretic Peptide Levels Improves the Prediction of Cardiovascular Event in Outpatients With Hypertension. (2015)
  • Author(s): Matsui S, Ishii J, Okuyama R, Takahashi H, Kawai H, Muramatsu T, Yamada A, Motoyama S, Naruse H, Hayashi M, Izawa H, Ozaki Y.
  • Organizer: Scientific Sessions 2015, American Heart Association.
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2015-11-07
  • Int'l Joint Research
• [Presentation] Prognostic Value of Combined Assessment of Oxidized Lipoprotein (a) and Estimated Glomerular Filtration Rate in Patients Undergoing Elective Coronary Angiography for Suspected Coronary Artery Disease. (2015)
  • Author(s): Naruse H, Ishii J, Okuyama R, Ohta M, Kawai H, Okumura M, Muramatsu T, Hayashi M, Matsui S, Motoyama S, Izawa H, Ozaki Y.
  • Organizer: Scientific Sessions 2015, American Heart Association.
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2015-11-07
  • Int'l Joint Research
• [Presentation] 心不全と糖尿病 (2015)
  • Author(s): 井澤英夫
  • Organizer: 第1回日本心臓リハビリテーション学会東海地方会
  • Place of Presentation: 岐阜市、じゅうろくプラザ
  • Year and Date: 2015-10-18
  • Invited
• [Presentation] 心不全の心臓リハビリテーション (2015)
  • Author(s): 井澤英夫
  • Organizer: 第38回日本リハビリテーション医学会九州地方会
  • Place of Presentation: 那覇市、てんぶす那覇
  • Year and Date: 2015-10-04
  • Invited
• [Presentation] 心不全の長期予後と心臓リハビリテーション (2015)
  • Author(s): 井澤英夫、河野裕治、粥川知子、青柳陽一郎、才藤栄一、吉川大治、藤原稚也、山田純生
  • Organizer: 第21回日本心臓リハビリテーション学会学術集会
  • Place of Presentation: 福岡市、福岡国際会議場
  • Year and Date: 2015-07-18
  • Invited
• [Presentation] Combination of Admission Urinary Albumin and Neutrophil Gelatinase-Associated Lipocalin Improves Prediction of Acute Kidney Injury in Patients Hospitalized to CCUs. (2015)
  • Author(s): Tousei Hashimoto, Junichi Ishii, Ryunosuke Okuyama, Hiroyuki Naruse, Yoshihisa Mori, Shigeru Matsui, Hideo Izawa, Yukio Ozaki.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Combination of High-Sensitive Troponin and N-Terminal Pro-B-Type Natriuretic Peptide Improves Prediction of Future Admission for Heart Failure in Hypertensive Patients. (2015)
  • Author(s): Junichi Ishii, Ryunosuke Okuyama, Hideki Kawai, Takashi Muramatsu, Hiroyuki Naruse, Sadako Matoyama, Shigeru Matsui, Eiichi Watanabe, Hideo Izawa, Yukio Ozaki.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Predictors for In-hospital Clinical Outcome in Patients Prescribed Tolvaptan for Their Worsening Heart Failure. (2015)
  • Author(s): Takehiro Ito, Daiji Yoshikawa, Mutsuharu Hayashi, Wakaya Fujiwara, Daisuke Mukaide, Yoshinori Sugishita, Toyoaki Murohara, Junichi Ishii, Yukio Ozaki, Hideo Izawa.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Combined Assessment of High-Sensitive Troponin I and NT-proBNP Improves Prediction of Future Admission for Heart Failure Outpatients with Hypertension. (2015)
  • Author(s): Shigeru Matsui, Junichi Ishii, Ryunosuke Okuyama, Masanori Okuyama, Tousei Hashimoto, Hideki Kawai, Takashi Muramatsu, Hiroyuki Naruse, Yoshihisa Mori, Sadako Motoyama, Hideo Izawa, Yukio Ozaki.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Combined Assessment of High-Sensitive Troponin I and NT-proBNP Improves Prediction of Future Admission for Heart Failure in Outpatients with CKD. (2015)
  • Author(s): Yoshihisa Mori, Junichi Ishii, Hiroshi Takahashi, Ryunosuke Okuyama, Tousei Hashimoto, Hideki Kawai, Takashi Muramatsu, Hiroyuki Naruse, Sadako Motoyama, Shigeru Matsui, Midori Hasegawa, Hideo Izawa, Yukio Yuzawa, Yukio Ozaki.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Combined Assessment of Plasma D-Dimer and Estimated GFR Improves Prediction of Mortality in Patients with Suspected Coronary Artery Disease. (2015)
  • Author(s): Hiroyuki Naruse, Junichi Ishii, Ryunosuke Okumura, Masaya Ohta, Masanori Okumura, Takashi Muramatsu, Shigeru Matsui, Hideo Izawa, Yukio Ozaki.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Combined Assessment of Serum Neutrophil Gelatinase-Associated Lipocalin and NT-proBNP on Admission Improves Prediction of Mortality in Patients Hospitalized to CCUs. (2015)
  • Author(s): Ryunosuke Okuyama, Junichi Ishii, Tousei Hashimoto, Hideki Kawai, Takashi Muramatsu, Hiroyuki Naruse, Shigeru Matsui, Sadako Motoyama, Yoshihisa Mori, Hideo Izawa, Yukio Ozaki.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Inhibitory Factors for a Prescription of Novel Oral Anti-Coagulant Agents for Non-Valvular Atrial Fibrillation: An Analysis from the ‘Real World’. (2015)
  • Author(s): Yoshinori Sugishita, Daiji Yoshikawa, Takehiro Ito, Tomohito Kamada, Masataka Yoshinaga, Daisuke Mukaide, Wakaya Fujiwara, Hiroatsu Yokoi, Mutsuharu Hayashi, Toyoaki Murohara, Yukio Ozaki, Hideo Izawa.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] Plasma Indoxyl Sulfate Levels Predict Long-term Clinical Outcomes in Patients with Coronary Artery Disease. (2015)
  • Author(s): Daiji Yoshikawa, Hideki Ishii, Susumu Suzuki, Kyosuke Takeshita, Mutsuharu Hayashi, Hideo Izawa, Yukio Ozaki, Toyoaki Murohara.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
  • Year and Date: 2015-04-24
  • Int'l Joint Research
• [Presentation] 糖尿病患者における心不全入院リスクと血清高感度トロポニンI濃度との関係 (2014)
  • Author(s): 成瀬寛之、石井潤一、奥山龍之介、太田将也、橋本踏青、奥村雅徳、森義久、村松崇、松井茂、 井澤英夫、鈴木敦詞、伊藤光泰、尾崎行男
  • Organizer: 第62回日本心臓病学会学術集会
  • Place of Presentation: 仙台、仙台国際センター
  • Year and Date: 2014-09-26 – 2014-09-28
• [Presentation] 高感度トロポニンI入院時測定の臨床的意義：冠動脈集中治療室 (CCU) における後ろ向き検討 (2014)
  • Author(s): 奥山龍之介、石井潤一、太田将也、奥村雅徳、成瀬寛之、松井茂、井澤英夫、尾崎行男
  • Organizer: 第62回日本心臓病学会学術集会
  • Place of Presentation: 仙台、仙台国際センター
  • Year and Date: 2014-09-26 – 2014-09-28
• [Presentation] 下半身陽圧器(LBPP)を用いた高齢者高血圧患者における左室拡張予備能障害の評価 (2014)
  • Author(s): 良永真隆、向出大介、石黒智也、山田亮、鎌田智仁、杉下義倫、藤原稚也、横井博厚、林睦晴、井澤英夫
  • Organizer: 第25回日本心エコー図学会学術集会
  • Place of Presentation: 金沢、ポルテ金沢
  • Year and Date: 2014-04-17 – 2014-04-19
• [Presentation] Glomerular Filtration Rate Estimated by Cystatin C- versus Creatinine-Based Equations for Predicting Cardiovascular Mortality in Patients Hospitalized for Worsening Heart Failure Without Advanced Renal Impairment (2013)
  • Author(s): Hiroyuki Naruse, Junichi Ishii, Ryuunosuke Okuyama, Masa Junichi Ishii, Ryuunosuke Okuyama, Masaya Ohta, Tousei Hashimoto, Kosuke Hattori, Masanori Okumura, Sadako Motoyama, Shigeru Matsui, Hideo Izawa, Yukio Ozaki
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: 米国 ダラス
• [Presentation] Multiple Biomarkers Improve the Prediction of Cardiovascular Mortality in Patients on Chronic Hemodialysis (2013)
  • Author(s): Junichi Ishii, Ryuunosuke Okuyama, Tousei Hashimoto, Kosuke Hattori, Hideki Kawai, Masanori Okumura, Hiroyuki Naruse, Sadako Motoyama, Shigeru Matsui, Hiroshi Takahashi, Toru Aoyama, Daisuke Kamoi, Hideo Izawa, Yukio Ozaki
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: 米国 ダラス
• [Presentation] Prognostic Value of Combination of High-Sensitivity Troponin T and B-Type Natriuretic Peptide in Outpatients With Type 2 Diabetes Mellitus (2013)
  • Author(s): Shigeru Matsui, Junichi Ishii, Atsushi Suzuki, Ryuunosuke Okuyama, Kosuke Hattori, Tousei Hashimoto, Hiroyuki Naruse, Sadako Motoyama, Mitsuyasu Ito, Hideo Izawa, Yukio Ozaki
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: 米国 ダラス
• [Presentation] Glomerular Filtration Rate Estimated by Cystatin C-Based versus Creatinine-Based Equations for Predicting Mortality in Outpatients With Type 2 Diabetes Mellitus (2013)
  • Author(s): Ryuunosuke Okuyama, Junichi Ishii, Atsushi Suzuki, Tousei Hashimoto, Kosuke Hattori, Hideki Kawai, Masanori Okumura, Hiroyuki Naruse, Sadako Motoyama, Shigeru Matsui, Hideo Izawa, Mitsuyasu Ito, Yukio Ozaki
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: 米国 ダラス
• [Presentation] Prognostic Value of Serum Levels of Neutrophil Gelatinase-Associated Lipocalin on Admission in Patients Hospitalized to Coronary Care Units (2013)
  • Author(s): Tousei Hashimoto, Junnichi Ishii, Ryunosuke Okuyama, Kousuke Hattori, Masanori Okumura, Hiroyuki Naruse, Shigeru Matsui, Sadako Motoyama, Hideo Izawa, Yukio Ozaki
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: 米国 ダラス
• [Presentation] 下半身陽圧器により評価した高齢者における左室拡張機能予備能 (2013)
  • Author(s): 向出大介、山田亮、石黒智也、鎌田智仁、杉下義倫、木下幸輔、藤原稚也、横井博厚、井澤英夫
  • Organizer: 第61回日本心臓病学会学術集会
  • Place of Presentation: 熊本
• [Presentation] 下半身陽圧器(LBPP)を用いて評価した高齢者における左室拡張予備能障害 (2013)
  • Author(s): 向出大介、石黒智也、山田亮、畳陽祐、鎌田智仁、杉下義倫、藤原稚也、横井博厚、井澤英夫
  • Organizer: 第50回記念日本臨床生理学会総会
  • Place of Presentation: 東京
• [Presentation] Serum NT-proBNP level correlated with left atrial dimension, not with left ventricular dimension in patients with chronic atrial fibrillation (2013)
  • Author(s): Wakaya Fujiwara, Hiroatsu Yokoi, Mutsuharu Hayashi, Daisuke Mukaide, Yoshinori Sugishita, Tomohito Kamada, Tomoya Ishiguro, Ryo Yamada, Hideo Izawa
  • Organizer: 第17回日本心不全学会学術集会
  • Place of Presentation: さいたま市