The molecular mechanism of blood cell-cell interaction forming niche inducing CD34+ cell expansion and differentiation to endothelial progenitor cells in vascular regeneration
| Project/Area Number |
25461091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ASAHARA Takayuki 東海大学, 医学部, 教授 (20246200)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 血管内皮前駆細胞 / CD34 / Notchシステム / 血管再生 / 単球 / 血液細胞間ニッチ / Notch リガンド / Notchリガンド |
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| Outline of Final Research Achievements |
We investigated blood cell-cell interactions to promote the expansion and differentiation of endothelial progenitor cells (EPCs) derived from CD34+ cells using the novel culture system developed by our laboratory. Peripheral blood mononuclear cells are divided into CD34+ stem cells, and CD34- cells of T lymphocytes (CD3+ cells), and monocytes (CD14+ cells). We found the promoting effect of CD34- cell populations on vascular regeneration capability of CD34+ cells via expansion and differentiation to endothelial progenitor cells (EPCs). Further, CD14+ cells turned out to be a key cell population responsible for the effect through a direct cell-cell interaction of Notch ligand/receptor system, Delta like -1 expressed on CD14+ cells to Notch receptor-1, or 2 on CD34+ cells, but not CD3+ cells.
The results indicate that CD34+ cells together with CD14+ cells form the functional ‘niche’ playing a critical role in vascular regeneration, providing a novel information in vascular biology.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.2014
Author(s)
H. Masuda, R. Tanaka, S. Fujimura, M. Ishikawa, H. Akimaru, T. Shizuno, A. Sato, Y. Okada, Y. Iida, J. Itoh, Y. Itoh, H. Kamiguchi, A. Kawamoto, T. Asahara.
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Journal Title
J Am Heart Assoc.
Volume: 3
Issue: 3
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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