| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We investigated blood cell-cell interactions to promote the expansion and differentiation of endothelial progenitor cells (EPCs) derived from CD34+ cells using the novel culture system developed by our laboratory. Peripheral blood mononuclear cells are divided into CD34+ stem cells, and CD34- cells of T lymphocytes (CD3+ cells), and monocytes (CD14+ cells). We found the promoting effect of CD34- cell populations on vascular regeneration capability of CD34+ cells via expansion and differentiation to endothelial progenitor cells (EPCs). Further, CD14+ cells turned out to be a key cell population responsible for the effect through a direct cell-cell interaction of Notch ligand/receptor system, Delta like -1 expressed on CD14+ cells to Notch receptor-1, or 2 on CD34+ cells, but not CD3+ cells.
The results indicate that CD34+ cells together with CD14+ cells form the functional ‘niche’ playing a critical role in vascular regeneration, providing a novel information in vascular biology.
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