| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
The aim of this study was to examine the role of ADAMTS in cardiovascular disease. We used ADAMTS knockout mouse in this study. First, we produced ADAMTS4/ADAMTS5 double knockout mouse. Because ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2) share the substrates, one of these two molecule may compensate the biological function in a single knockout mouse, we decided to produce double knockout mouse.
The double knockout mice were survived and there was no dwarfism observed. There was no particular abnormality in the tissue development. We then cross double knockout mice with ApoE knockout mouse. ApoE knockout mouse is used for atherosclerosis study. We succeeded to produced triple knockout mice and we gave them high-fat diet. It was interesting that there was a significant difference in the development of atherosclerosis between ApoE single and triple knockout mice.
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