Establishment of the method to differentiate human cardiac stem cells into cardiomyocytes
| Project/Area Number |
25461118
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Tokai University |
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Principal Investigator |
HOSODA Toru 東海大学, 創造科学技術研究機構, 准教授 (50608601)
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| Research Collaborator |
TOMOIKE Hitonobu 榊原記念病院, 院長 (90112333)
IGUCHI Nobuo 榊原記念病院, 循環器内科放射線科, 部長 (90232165)
TAKANASHI Shuichiro 榊原記念病院, 心臓血管外科, 主任部長 (30206776)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 心臓幹細胞 / 心筋分化 / 心不全 / 体性幹細胞 / 心筋分化誘導 / 再生医学 |
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| Outline of Final Research Achievements |
Human c-kit-positive cardiac stem cells (CSCs) renew cardiomyocytes and coronary vessels maintaining the homeostasis of the organ. This study aimed to establish the method to differentiate CSCs into cardiomyocytes in vitro.
Referring to preceding researches, physiologically active substances were applied to CSCs in various combinations, but myocardial differentiation was not achieved. Accordingly, we altered our approach; CSCs and human induced pluripotent stem cells (iPSCs) were co-cultured without a direct contact each other using the Transwell system and treated with an established condition for the myocardial differentiation of iPSCs. As a result, iPSCs and CSCs started to beat on day 14 and 30, respectively, and continued for at least 2 weeks.
Beating cells were prepared from CSCs in vitro for the first time. By identifying the critical factor(s) involved in the process, we can expect to obtain more physiological cardiomyocytes with high efficiency compared to former approaches.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Response to letter regarding article "inositol 1,4,5-trisphosphate receptors and human left ventricular myocytes".2014
Author(s)
Signore S, Sorrentino A, Ferreira-Martins J, Kannappan R, Shafaie M, Del Ben F, Isobe K, Arranto C, Wybieralska E, Webster A, Sanada F, Ogórek B, Zheng H, Liu X, Del Monte F, D'Alessandro DA, Wunimenghe O, Michler RE, Hosoda T, Goichberg P, Leri A, Kajstura J, Anversa P, Rota M.
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Journal Title
Circulation
Volume: 129
Issue: 21
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] c-Kit-positive cardiac stem cells nested in hypoxic niches are activated by stem cell factor reversing the aging myopathy.2014
Author(s)
Sanada F, Kim J, Czarna A, Chan NY, Signore S, Ogorek B, Isobe K, Wybieralska E, Borghetti G, Pesapane A, Sorrentino A, Mangano E, Cappetta D, Mangiaracina C, Ricciardi M, Cimini M, Ifedigbo E, Perrella MA, Goichberg P, Choi AM, Kajstura J, Hosoda T, Rota M, Anversa P, Leri A.
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Journal Title
Circulation Research
Volume: 114
Issue: 1
Pages: 41-55
DOI
Related Report
Peer Reviewed
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[Journal Article] Inositol 1, 4, 5-trisphosphate receptors and human left ventricular myocytes.2013
Author(s)
Signore S, Sorrentino A, Ferreira-Martins J, Kannappan R, Shafaie M, Del Ben F, Isobe K, Arranto C, Wybieralska E, Webster A, Sanada F, Ogorek B, Zheng H, Liu X, Del Monte F, D'Alessandro DA, Wunimenghe O, Michler RE, Hosoda T, Goichberg P, Leri A, Kajstura J, Anversa P, Rota M.
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Journal Title
Circulation
Volume: 128
Issue: 12
Pages: 1286-1297
DOI
Related Report
Peer Reviewed
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