The role of JCAD, an adhesion molecule of endothelial cell, in regulating cardiovascular disease
Project/Area Number |
25461129
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cardiovascular medicine
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Research Institution | Kobe University |
Principal Investigator |
kawai hiroya 神戸大学, 医学(系)研究科(研究院), 教授 (20346266)
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Co-Investigator(Kenkyū-buntansha) |
ISHIDA TATSUEO 神戸大学, 医学研究科, 特命教授 (00379413)
杜 隆嗣 神戸大学, 医学(系)研究科(研究院), 准教授 (50379418)
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Co-Investigator(Renkei-kenkyūsha) |
TOH RYUJI 神戸大学, 医学研究科, 特命准教授 (50379418)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | JCAD / angiogenesis / 血管内皮細胞 / 循環器 / 血管新生 / 動脈硬化 / 接着分子 / VE-カドヘリン |
Outline of Final Research Achievements |
Cell culture experiments revealed impaired angiogenic ability (proliferation, migration, and tube formation) by the knockdown of JCAD with siRNA. We have generated mice lacking JCAD (mKIAA1462 -/-) by gene-targeted deletion of JCAD to address in vivo angiogenic function. mKIAA1462-/- mice did not show morphological differences in development of retinal vasculature. Ex vivo aortic ring model demonstrated impaired neovascularization in aorta from mKIAA1462-/- mice than control wild-type mice (p<0.05). Tumor growth was assessed by monitoring tumor volume after the subcutaneous injection of melanoma cells into the mice. mKIAA1462-/- mice exhibited 45% smaller tumor volume compared with wild-type mice (p<0.001). Histological assessment of the tumor exhibited less smooth muscle actin (SMA)-positive neovascularization determined by CD31 staining in tumor of mKIAA1462-/- mice than wild-type mice, indicating that knockdown of JCAD inhibited the vascular maturation in angiogenic process.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Trans-fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll-like receptors.2014
Author(s)
Kondo K, Ishida T, Yasuda T, Nakajima H, Mori K, Tanaka N, Mori T, Monguchi T, Shinohara M, Irino Y, Toh R, Rikitake Y, Kiyomizu K, Tomiyama Y, Yamamoto J, Hirata K.
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Journal Title
Molecular Nutrition & Food Research
Volume: 59
Issue: 4
Pages: 729-740
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Endothelial Lipase Modulates Pressure Overload- Induced Heart Failure Through Alternative Pathway for Fatty Acid Uptake.2013
Author(s)
Nakajima H, Ishida T, Satomi-Kobayashi S, Mori K, Hara T, Sasaki N, Yasuda T, Toh R, Tanaka H, Kawai H, Hirata K
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Journal Title
Hypertension
Volume: 61(5)
Issue: 5
Pages: 1002-7
DOI
Related Report
Peer Reviewed
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[Presentation] Trans-fatty acid accelerates thrombus formation in vivo through aggravating anti-thrombogenic phenotype of vascular endothelial cells.2013
Author(s)
Kensuke Kondo, Tatsuro Ishida, Tomoyuki Yasuda, Junichiro Yamamoto, Nobuaki Tanaka, Takeshige Mori, Tomoko Monguchi, Kenta Mori, Hideto Nakajima, Ryuji Toh, Kenichi Hirata.
Organizer
Amerikan Heart Association Scientifi Sessions 2013
Place of Presentation
Dallas, USA
Related Report
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