Effect of respiratory disease onset and functionality in the alveolar-bronchial of oxidative stress defense protein DJ-1
Project/Area Number |
25461184
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Respiratory organ internal medicine
|
Research Institution | Toho University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 酸化ストレス / DJ-1タンパク質 / 慢性閉塞性肺疾患 / 薬物輸送担体 / 慢性閉塞生肺疾患 / 炎症 / シグナル伝達 / 細胞死 / 薬物輸送体 |
Outline of Final Research Achievements |
I examined the involvement of the function of the lungs and oxidative stress defense protein DJ-1 respiratory disease onset. Using human lung epithelial cell-derived A549, to establish a DJ-1 protein knockdown cell line. As a result, the knockdown cell lines, became more vulnerable to oxidative stress. Then, the gene whose expression is varied among A549 cells and knockdown cell lines were screened by DNA micro array. As a result, gene expression of some transporters were varied. The transporter involved in respiratory disease onset have been reported. The transporter and DJ-1 are co-localization is revealed from confocal microscopy. Coordinated action of DJ-1 protein and the transporter was suggested.
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Report
(4 results)
Research Products
(1 results)