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Development of a novel vaccine therapy using nanoparticles and cross-presentation machinery

Research Project

Project/Area Number 25461186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Enomoto Noriyuki  浜松医科大学, 医学部附属病院, 講師 (50436961)

Co-Investigator(Kenkyū-buntansha) Suda Takafumi  浜松医科大学, 医学部, 教授 (30291397)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords気管支喘息 / ワクチン療法 / ナノ粒子 / 樹状細胞 / 細胞障害性T細胞 / クロスプレゼンテーション
Outline of Final Research Achievements

In this study, nanoparticle, which consists of polylactic coglycolic acid (PLGA), was used to induce allergen specific cytotoxic T-lymphocytes (CTL). PLGA facilitates the induction of CTL via cross-presentation machinery in antigen-presenting cells (APC), and these CTL can kill dendritic cell (DC) that plays a role in bronchial asthma.
We found PLGA efficiently induced ovalbumin (OVA)-specific CTL via antigen-presenting cells in vitro. Next, PLGA/OVA was intranasally administered to OVA-induced asthma model mice. PLGA/OVA reduced eosinophil number in bronchoalveolar lavage compared to that after treatment with OVA alone. However, PLGA/OVA could not significantly reduce eosinophil number or induce allergen-specific CTL compared to those of untreated mice. There is a possibility that more manipulation of PLGA/OVA is necessary to improve the delivery and phagocytosis of allergen in APC and to facilitate cross-presentation in vivo.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Amount of elastic fibers predicts prognosis of idiopathic pulmonary fibrosis.2013

    • Author(s)
      Enomoto N, Suda T, Kono M, Kaida Y, Hashimoto D, Fujisawa T, Inui N, Nakamura Y, Imokawa S, Funai K, Chida K.
    • Journal Title

      Respir Med.

      Volume: 107 Issue: 10 Pages: 1608-1616

    • DOI

      10.1016/j.rmed.2013.08.008

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Idiopathic pleuroparenchymal fibroelastosis (IPPFE): Comparison of clinico-pathological findings with idiopathic pulmonary fibrosis (IPF)2013

    • Author(s)
      Noriyuki Enomoto
    • Organizer
      Annual meeting of American Thoracic Society
    • Place of Presentation
      Philadelphia
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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