The role of metallothionein in diabetic nephropathy
Project/Area Number |
25461223
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Kidney internal medicine
|
Research Institution | Okayama University |
Principal Investigator |
Ogawa Daisuke 岡山大学, 医歯(薬)学総合研究科, 准教授 (70535195)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 糖尿病性腎症 / 酸化ストレス / 炎症 |
Outline of Final Research Achievements |
Metallothionein (MT) is induced in proximal tubular epithelial cells as an antioxidant in diabetic kidney; however, the role of MT in renal function remains unclear. We therefore investigated if MT deficiency accelerates diabetic nephropathy through oxidative stress and inflammation. Diabetes was induced by streptozotocin injection in MT-deficient (MT-/-) and MT+/+ mice. Murine proximal tubular epithelial cells were used to elucidate the role of MT under high-glucose conditions. Parameters of diabetic nephropathy and markers of ROS and inflammation were accelerated in diabetic MT-/- mice compared with diabetic MT+/+ mice. In vitro studies demonstrated that knockdown of MT by small interfering RNA enhanced mitochondrial ROS generation and inflammation-related gene expression in cultured cells under high-glucose conditions. These results suggest that MT may play a key role in protecting the kidney against high glucose-induced ROS and subsequent inflammation in diabetic nephropathy.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Identification of Circulating miR-101, miR-375 and miR-802 as Biomarkers for Type 2 Diabetes.2015
Author(s)
Higuchi C, Nakatsuka A, Eguchi J, Teshigawara S, Kanzaki M, Katayama A, Yamaguchi S, Takahashi N, Murakami K, Ogawa D, Sasaki S, Makino H, Wada J.
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Journal Title
Metabolism
Volume: 64(4)
Issue: 4
Pages: 489-97
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Translocase of inner mitochondrial membrane 44 alters the mitochondrial fusion and fission dynamics and protects from type 2 diabetes.2015
Author(s)
Wang Y, Katayama A, Terami T, Han X, Nunoue T, Zhang D, Teshigawara S, Eguchi J, Nakatsuka A, Murakami K, Ogawa D, Furuta Y, Makino H, Wada J.
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Journal Title
Metabolism
Volume: 未定
Issue: 6
Pages: 677-88
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Pemt deficiency ameliorates endoplasmic reticulum stress in diabetic nephropathy2014
Author(s)
Watanabe M, Nakatsuka A, Murakami K, Inoue K, Terami T, Higuchi C, Katayama A, Teshigawara S, Eguchi J, Ogawa D, Watanabe E, Wada J, Makino H
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Journal Title
PLoS ONE
Volume: 9(3)
Issue: 3
Pages: e92647-e92647
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Long-term treatment with the sodium glucose cotransporter 2 inhibitor, dapagliflozin, ameliorates glucose homeostasis and diabetic dephropathy in db/db mice.2014
Author(s)
Terami N, Ogawa D, Tachibana H, Hatanaka T, Wada J, Nakatsuka A, Eguchi J, Horiguchi CS, Nishii N, Yamada H, Takei K, Makino H.
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Journal Title
PLoS One
Volume: 9
Issue: 6
Pages: e100777-e100777
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Metallothionein deficiency exacerbates diabetic nephropathy in streptozotocin-induced diabetic mice2014
Author(s)
Tachibana H, Ogawa D, Sogawa N, Asanuma M, Miyazaki I, Terami N, Hatanaka T, Horigushi CS, Nakatsuka A, Eguchi J, Wada J, Yamada H, Takei K, Makino H
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Journal Title
Am J Physiol Renal Physiol
Volume: 306
Issue: 1
Pages: 105-115
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Urinary fetuin-A is a novel marker for diabetic neohropathy in type 2 diabetes identified by lectin microarray2013
Author(s)
Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, Ogawa D, Terami T, Katayama A, Tone A, Iseda I, Hida K, Yamada M, Ogawa T, Makino H
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Journal Title
PLoS ONE
Volume: 8(10)
Issue: 10
Pages: e77118-e77118
DOI
Related Report
Peer Reviewed
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