Analysis of AMPK for cell protective function in renal tubular epithelial cells

• Project/Area Number: 25461227
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Jichi Medical University
• Principal Investigator: Nagata Daisuke 自治医科大学, 医学部, 教授 (40393194)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: AMPK / アポトーシス / オートファジー / 腎間質線維化 / 腎尿細管 / AMPキナーゼ / 遺伝子改変マウス / 間質線維化

Outline of Final Research Achievements

Preliminary experiments conducted at Dokkyo Medical University, which the primary investigator Dr. Nagata was belonging to when this study started, revealed that interstitial fibrosis was enhanced in dnAMPK mice and decreased in caAMPK. On the other hand, on day 5 after tubule ligation, no significant difference could be detected on the 8th day. I planned to measure the relevant factors of apoptosis and autophagy at a different timing in Jichi Medical University, however it became clear that neither dnAMPK nor caAMPK can be induced with doxycycline specifically in the renal tubular epithelial cells. Although it is expected that some abnormality occurred during SPF conversion at the time of moving, I am now performing some basic troubleshooting. After this problem solving, I hope to further carry out the current tasks.

Research Products

• [Journal Article] Telmisartan activates endothelial nitric oxide synthase via Ser1177 phosphorylation in vascular endothelial cells (2014)
  • Author(s): Myojo M, Nagata D, Fujita D, Sato T, Kiyosue A, Takahashi M, Satonaka H, Morishita Y, Nagai R, Komuro I, Hirata Y
  • Journal Title: PLOS ONE Volume: 1 Pages: 1-10
  • Peer Reviewed
• [Book] 内科【慢性腎臓病(CKD)診療を極める】 (2016)
  • Author(s): 長田 太助, 瀧 史香, 前嶋 明人, 田中 哲洋
  • Total Pages: 169
  • Publisher: 南江堂
• [Book] 循環plus【動脈硬化と臓器障害】 高齢者CKDの特徴と管理 (2016)
  • Author(s): 長田太助
  • Total Pages: 6
  • Publisher: メディカルトリビューン
• [Remarks] 自治医科大学内科学講座腎臓内科学講座
  • URL: http://www.jichi.ac.jp/usr/neph/index.html